Team:Osaka/week8
From 2011.igem.org
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==September 21(Wed)== | ==September 21(Wed)== | ||
+ | # Miniprep of yesterday's culture | ||
+ | # Restriction digests of minipreppped parts | ||
+ | # Gel electrophoresis of digests | ||
+ | #* ok | ||
+ | # Ligation | ||
+ | #* 115(A):113(upstream)+114(downstream)+1-1G(vector) | ||
+ | # Transformation of ligation products | ||
# colony check (cell survival) | # colony check (cell survival) | ||
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# Plating (cell survival) | # Plating (cell survival) | ||
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Revision as of 03:03, 22 September 2011
Contents |
September 18(Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- To liquid culture:03,105,106,107,108,109,110,111,112
September 19(Mon)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Ligation
- 113(T):105(upstream)+110(downstream)+1-7A(vector)
- 114(T):111(upstream)+112(downstream)+1-7A(vector)
- Transformation of ligation products
- Plating (cell survival)
Name | OD600 | UV(mJ/cm^2) |
---|---|---|
PprI-1 | 0.0109 | 0 |
PprI-2 | 0.0109 | 0.5 |
PprI-3 | 0.0109 | 1.0 |
PprI-4 | 0.0109 | 2.0 |
PprI-5 | 0.0109 | 5.0 |
PprA-1 | 0.0418 | 0 |
PprA-2 | 0.0418 | 0.5 |
PprA-3 | 0.0418 | 1.0 |
PprA-4 | 0.0418 | 2.0 |
PprA-5 | 0.0418 | 5.0 |
PprM-1 | 0.0454 | 0 |
PprM-2 | 0.0454 | 0.5 |
PprM-3 | 0.0454 | 1.0 |
PprM-4 | 0.0454 | 2.0 |
PprM-5 | 0.0454 | 5.0 |
RecA-1 | 0.0114 | 0 |
RecA-2 | 0.0114 | 0.5 |
RecA-3 | 0.0114 | 1.0 |
RecA-4 | 0.0114 | 2.0 |
RecA-5 | 0.0114 | 5.0 |
empty vector-1 | 0.0515 | 0 |
empty vector-2 | 0.0515 | 0.5 |
empty vector-3 | 0.0515 | 1.0 |
empty vector-4 | 0.0515 | 2.0 |
empty vector-5 | 0.0515 | 5.0 |
wild type-1 | 0.0546 | 0 |
wild type-2 | 0.0546 | 0.5 |
wild type-3 | 0.0546 | 1.0 |
wild type-4 | 0.0546 | 2.0 |
wild type-5 | 0.0546 | 5.0 |
September 20(Tue)
- To liquid culture:113,114
- colony check (cell survival)
Name | UV(mJ/cm^2) | conoly counts |
---|---|---|
PprI-1 | 0 | |
PprI-2 | 0.5 | |
PprI-3 | 1.0 | |
PprI-4 | 2.0 | |
PprI-5 | 5.0 | |
PprA-1 | 0 | |
PprA-2 | 0.5 | |
PprA-3 | 1.0 | |
PprA-4 | 2.0 | |
PprA-5 | 5.0 | |
PprM-1 | 0 | |
PprM-2 | 0.5 | |
PprM-3 | 1.0 | |
PprM-4 | 2.0 | |
PprM-5 | 5.0 | |
RecA-1 | 0 | |
RecA-2 | 0.5 | |
RecA-3 | 1.0 | |
RecA-4 | 2.0 | |
RecA-5 | 5.0 | |
Empty vector-1 | 0 | |
Empty vector-2 | 0.5 | |
Empty vector-3 | 1.0 | |
Empty vector-4 | 2.0 | |
Empty vector-5 | 5.0 | |
wild type-1 | 0 | |
wild type-2 | 0.5 | |
wild type-3 | 1.0 | |
wild type-4 | 2.0 | |
wild type-5 | 5.0 |
- Plating (cell survival)
Name | OD600 | UV(mJ/cm^2) |
---|---|---|
PprI-1 | 0.0109 | 0 |
PprI-2 | 0.0109 | 1.0 |
PprI-3 | 0.0109 | 2.0 |
PprI-4 | 0.0109 | 3.0 |
PprI-5 | 0.0109 | 4.0 |
PprA-1 | 0.0418 | 0 |
PprA-2 | 0.0418 | 1.0 |
PprA-3 | 0.0418 | 2.0 |
PprA-4 | 0.0418 | 3.0 |
PprA-5 | 0.0418 | 4.0 |
PprM-1 | 0.0454 | 0 |
PprM-2 | 0.0454 | 1.0 |
PprM-3 | 0.0454 | 2.0 |
PprM-4 | 0.0454 | 3.0 |
PprM-5 | 0.0454 | 4.0 |
RecA-1 | 0.0114 | 0 |
RecA-2 | 0.0114 | 1.0 |
RecA-3 | 0.0114 | 2.0 |
RecA-4 | 0.0114 | 3.0 |
RecA-5 | 0.0114 | 4.0 |
empty vector-1 | 0.0515 | 0 |
empty vector-2 | 0.0515 | 1.0 |
empty vector-3 | 0.0515 | 2.0 |
empty vector-4 | 0.0515 | 3.0 |
empty vector-5 | 0.0515 | 4.0 |
wild type-1 | 0.0546 | 0 |
wild type-2 | 0.0546 | 1.0 |
wild type-3 | 0.0546 | 2.0 |
wild type-4 | 0.0546 | 3.0 |
wild type-5 | 0.0546 | 4.0 |
September 21(Wed)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Ligation
- 115(A):113(upstream)+114(downstream)+1-1G(vector)
- Transformation of ligation products
- colony check (cell survival)
- Plating (cell survival)