Team:UCL London/Research/Stresslights/Experiments

From 2011.igem.org

(Difference between revisions)
PhilippBoeing (Talk | contribs)
(Created page with "{{:Team:UCL_London/Template/Header}}<html> <div id="submenu" class="sm-research-stresslights"> <div id="research-supercoiliology"><a href="https://2011.igem.org/Team:UCL_London/Re...")
Newer edit →

Revision as of 01:59, 22 September 2011

Modifying Oximeter in the lab

Aim

To modify and improve the oximeter device (BBa_K239011) using modified nark promoter (BBa_K239006) submitted by 2009 UCL iGEM team. Changes included replacing the normal GFP (green fluorescent protein) with an enhanced YFP (yellow fluorescent protein), which has an LVA tag added to its C terminal.

Ucl-content-Oximieter-circuit.jpg

Parts used

Part Name

Parts  ID

Parts Description

Length (bp)

Plasmid Backbone

mNARK promoter

BBa_K239006

promoter sensitive to hypoxia

89

on pUC57 (2710 bp) - ampicillin resistance

RBS

BBa_B0034

ribosome binding site

12

on pSB1A2 (2079 bp) - ampicillin resistance

YFP.LVA

BBa_E0032

enhanced yellow fluorescent protein with LVA tag

759

on pSB1A2 (2079 bp)

Terminator

BBa_B0015

double terminator (B0010-B0012)

129

on pSB1A2 (2079 bp)

pSB1K3

n/a

standard plasmid backbone (kanamycin resistance)

2204

n/a

pSB1T3

n/a

standard plasmid backbone (tetracycline resistance)

2463

n/a

pSB1C3

n/a

standard plasmid backbone (chloramphenicol resistance)

2070

n/a

Retrieved from "http://2011.igem.org/Team:UCL_London/Research/Stresslights/Experiments"