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Team:Missouri Miners/Notebook
From 2011.igem.org
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Revision as of 00:36, 22 September 2011
Notebook
July
| Sunday | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday |
|---|---|---|---|---|---|---|
| 26 | 27 | 28 | 29
*Resuspension of Omp-R, eYFP, and RBS | 30 | 1 | 2 |
| 3 | 4 | 5
*Isolated
plasmids
| 6
*Mini Preps.
*Digest1
| 7
*Ran Gel for Digest1 (Gel Incon- clusive) | 8
*Re-did Digest1 and Gel1 *Cut out bands | 9 |
| 10 | 11
*Ectracted plasmid parts from gel *Ligation1 | 12
*Transformation of E.coli with lig.1 products *Digest2 (eYFP and RBS) *Gel of Digest2 *Extracted bands of Digest2 | 13
*Transform.1 had colonies *Re-did eYFP digest2 *Ran eYFP D2 gel *Extracted eYFP *Ligation2 | 14
*Transformation2 *Innoculated transform.1 into broth | 15
*Isolated plasmids from transform.1 | 16 |
| 17 | 18 | 19 | 20 | 21 | 22 | 23 |
| 24 | 25 | 26 | 27 | 28 | 29 | 30 |
