LasR Autoinducing Loops
Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.
Leakage of the LasR autoinducing loops
To estimate the autoactivation of the LasR loop through a leaky promoter we measured fluorescence of cells carrying an in- and output element ( different constructs of the LasR autoinducing loop family and :
| | no-tag | DAS | LVA |
|
| RBS 0,07 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607031 BBa_K607031]
|
|
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607027 BBa_K607027]
|
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607023 BBa_K607023]
| 
|
| RBS 0,3 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607032 BBa_K607032]
|
|
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607028 BBa_K607028]
|
|
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607024 BBa_K607024]
|
|
| RBS 0,6 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607033 BBa_K607033]
|
|
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607029 BBa_K607029]
|
|
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607025 BBa_K607025]
|
|
| RBS 1 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607034 BBa_K607034]
|
|
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607030 BBa_K607030]
|
|
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607026 BBa_K607026]
|
|
| Figure 1. Flow cytometer data obtained at different time-points during exponential growth of cells carrying different LasR autoinducing loops in presence of 100 nM HSL.
|
|
"
