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Team:Groningen/project characterisation mu lasr
From 2011.igem.org
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| RBS 0,07 || <gallery>File:lasR9.png | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607031 BBa_K607031] </gallery> || <gallery>File:lasR5.png | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607027 BBa_K607027] </gallery> | | RBS 0,07 || <gallery>File:lasR9.png | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607031 BBa_K607031] </gallery> || <gallery>File:lasR5.png | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607027 BBa_K607027] </gallery> | ||
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|rowspan="4"| [[File:legenda.png]] | |rowspan="4"| [[File:legenda.png]] | ||
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Revision as of 23:19, 21 September 2011
LasR Autoinducing Loops
Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.
Leakage of the LasR autoinducing loops
To estimate the autoactivation of the LasR loop through a leaky promoter we measured fluorescence of cells carrying the following constructs:
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607023 BBa_K607023] | 
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| Figure 1. Flow cytometer data obtained at different time-points during exponential growth of cells carrying different LasR autoinducing loops in presence of 100 nM HSL. | ||||||
