Team:Glasgow/Results/MCS
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+ | The part is still awaiting synthesis.<br/><br/> | ||
- | < | + | You can visit the Multiple Cloning Site Part Page <a href=https://2011.igem.org/Team:Glasgow/mcs>here</a> |
Revision as of 23:15, 21 September 2011
![](http://farm7.static.flickr.com/6122/5958857609_b9df31d718_b.jpg)
Aims
- To produce an easier method for testing large numbers of biobricks- To reduce the overall number of ligations needed to do this
Method
The restriction enzymes we chose to include in our Multiple Cloning Site are XhoI, BamHI, SalI, BglI and HindIII.![](https://static.igem.org/mediawiki/2011/0/00/Glasgowmcsonly.png)
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:
Forward Primer | 5'-aattcgcggccgcttctagagggtaccggcgccctcgagggatcca agcttTactagagaaaaaaaaaccccgcccctgacagggcggggttttt ttttactagtagcggccgctgca-3' |
Reverse Primer | 5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCTCTAGAAGCGGCCGCG-3' |
You can visit the Multiple Cloning Site Part Page here