Team:Glasgow/Results/MCS
From 2011.igem.org
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Aims
Back to Results
- To develop a more efficient method for testing large numbers of biobricksMethod
The restriction enzymes we chose to include in our Multiple Cloning Site are XhoI, BamHI, SalI, BglI and HindIII.![](https://static.igem.org/mediawiki/2011/0/00/Glasgowmcsonly.png)
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:
Forward Primer | 5'-AATTCGCGGCCGCTTCTAGAGGGTACCGGCGCCCTCGAGGGTCCAGCTTTACTAGAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTT-3' |
Reverse Primer |
5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACC CTCTAGAAGCGGCCGCG-3' |
The part is still awaiting synthesis.
You can visit the Multiple Cloning Site Part Page here