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Team:EPF-Lausanne/Our Project/T7 promoter variants/t7prom/making
From 2011.igem.org
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== Gene-Specific PCR == | == Gene-Specific PCR == | ||
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| + | The first PCR that is used to build a full T7 promoter variant is called a "gene-specific" PCR. The primers are chosen so as to add a ribosome-binding site (rbs) upstream of the reporter gene (here RFP or Lysis) and a terminator downstream of the gene. It is in effect a typical PCR. | ||
[[File:rbs_lys_term.png]] | [[File:rbs_lys_term.png]] | ||
Revision as of 23:00, 21 September 2011
The Making of a T7 Promoter Variant
Gene-Specific PCR
The first PCR that is used to build a full T7 promoter variant is called a "gene-specific" PCR. The primers are chosen so as to add a ribosome-binding site (rbs) upstream of the reporter gene (here RFP or Lysis) and a terminator downstream of the gene. It is in effect a typical PCR.
Extension PCR: Part I
Extension PCR: Part II
