Team:Groningen/project characterisation mu lasr

From 2011.igem.org

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==Leakiness of the LasR autoinducing loops==
==Leakiness of the LasR autoinducing loops==
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Revision as of 22:30, 21 September 2011


LasR Autoinducing Loops

Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.

Lasr2.png

Leakiness of the LasR autoinducing loops

File:LasR1.png LasR2.png LasR3.png LasR4.png LasR5.png LasR6.png File:LasR7.png LasR8.png LasR9.png LasR10.png LasR11.png LasR12.png


no-tag DAS LVA
RBS 0,07

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607031 BBa_K607031]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607027 BBa_K607027]

LasR1.png

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607023 BBa_K607023]

Legenda.png
RBS 0,3

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607028 BBa_K607028]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607024 BBa_K607024]

RBS 0,6
RBS 1
Figure 4. Flow cytometer data taken at different time-points during exponential growth of cells carrying different cI autoinducing loops.




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