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Team:Groningen/project characterisation mu lasr
From 2011.igem.org
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| || no-tag|| DAS || LVA || | | || no-tag|| DAS || LVA || | ||
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| - | | RBS 0,07 || <gallery> | + | | RBS 0,07 || <gallery>File:lasR9.png | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607031 BBa_K607031] </gallery> || <gallery>File:lasR5.png | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607027 BBa_K607027] </gallery> |
| - | || <gallery> | + | || <gallery>File:lasR1.png | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607023 BBa_K607023] </gallery> |
|rowspan="4"| [[File:legenda.png]] | |rowspan="4"| [[File:legenda.png]] | ||
|- | |- | ||
| - | | RBS 0,3 || <gallery>[[File:lasR10.png]]| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607032 BBa_K607032]</gallery> || <gallery> | + | | RBS 0,3 || <gallery>[[File:lasR10.png]]| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607032 BBa_K607032]</gallery> || <gallery>File:lasR6.png| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607028 BBa_K607028]</gallery> || <gallery>File:lasR2.png| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607024 BBa_K607024]</gallery> |
|- | |- | ||
| RBS 0,6 || <gallery> [[File:lasR11.png]]|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607033 BBa_K607033] </gallery> | | RBS 0,6 || <gallery> [[File:lasR11.png]]|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607033 BBa_K607033] </gallery> | ||
Revision as of 22:27, 21 September 2011
LasR Autoinducing Loops
Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.
Leakiness of the LasR autoinducing loops
| no-tag | DAS | LVA | |||||
| RBS 0,07 |
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| RBS 0,3 | |
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| RBS 0,6 | | | | ||||
| RBS 1 | | | | ||||
| Figure 4. Flow cytometer data taken at different time-points during exponential growth of cells carrying different cI autoinducing loops. | |||||||
