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Revision as of 22:04, 21 September 2011 (view source) HannahRalph (Talk | contribs) ← Older edit		Revision as of 22:06, 21 September 2011 (view source) HannahRalph (Talk | contribs) Newer edit →
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	<tr>		<tr>
	<td>Reverse Primer</td>		<td>Reverse Primer</td>
-	<td>5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG	+	<td>5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG                 TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCTCTAGAAGCGGCCGCG-3'</td>
-		+
-	TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCT	+
-		+
-	CTAGAAGCGGCCGCG-3'</td>	+
	</tr>		</tr>
	</table>		</table>

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Revision as of 22:06, 21 September 2011

Aims

- To produce an easier method for testing large numbers of biobricks
- To reduce the overall number of ligations needed to do this

Method

The restriction enzymes we chose to include in our Multiple Cloning Site are XhoI, BamHI, SalI, BglI and HindIII.



We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:

Forward Primer	5'-aattcgcggccgcttctagagggtaccggcgccctcgagggatcca agcttTactagagaaaaaaaaaccccgcccctgacagggcggggttttt ttttactagtagcggccgctgca-3'
Reverse Primer	5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCTCTAGAAGCGGCCGCG-3'

Results