Team:Glasgow/Results/MCS
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<center><img src=https://static.igem.org/mediawiki/2011/0/00/Glasgowmcsonly.png></center> | <center><img src=https://static.igem.org/mediawiki/2011/0/00/Glasgowmcsonly.png></center> | ||
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- | We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below: | + | We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:<br/><Br/> |
<u>Forward Primer</u><br/><br/> | <u>Forward Primer</u><br/><br/> |
Revision as of 21:59, 21 September 2011
![](http://farm7.static.flickr.com/6122/5958857609_b9df31d718_b.jpg)
Aims
- To produce an easier method for testing large numbers of biobricks- To reduce the overall number of ligations needed to do this
Method
The restriction enzymes we chose to include in our Multiple Cloning Site are XhoI, BamHI, SalI, BglI and HindIII.![](https://static.igem.org/mediawiki/2011/0/00/Glasgowmcsonly.png)
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:
Forward Primer
5'-aattcgcggccgcttctagagggtaccggcgccctcgagggatcca agcttTactagagaaaaaaaaaccccgcccctgacagggcggggttttt ttttactagtagcggccgctgca-3'
Reverse Primer
5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCT CTAGAAGCGGCCGCG-3'