Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs
From 2011.igem.org
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| P39QY42M ||site directed||SP6||eGFP||plasmid||worked well||X||<html> <a href="http://partsregistry.org/Part:BBa_K613019">BBa_K613019</a> </html>|||| | | P39QY42M ||site directed||SP6||eGFP||plasmid||worked well||X||<html> <a href="http://partsregistry.org/Part:BBa_K613019">BBa_K613019</a> </html>|||| | ||
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- | | <html> <a href="http://partsregistry.org/Part:BBa_K613020"> | + | | P39QY42ML197S||PCR-induced||T7||His-tag||linear ||ITT tested||||<html> <a href="http://partsregistry.org/Part:BBa_K613020">BBa_K613020</a></html>|||| |
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- | | <html> <a href="http://partsregistry.org/Part:BBa_K613021"> | + | | P39QY42ML52P||PCR-induced||T7||His-tag||linear ||ITT tested||||<html> <a href="http://partsregistry.org/Part:BBa_K613021">BBa_K613021</a></html>|||| |
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- | | <html> <a href="http://partsregistry.org/Part:BBa_K613022"> | + | | E37AP39K||site directed||T7||His-tag||linear ||||||<html> <a href="http://partsregistry.org/Part:BBa_K613022">BBa_K613022</a></html>||X|| |
|- | |- | ||
- | | <html> <a href="http://partsregistry.org/Part:BBa_K613023"> | + | | E37AP39QY42F||site directed||T7||His-tag||linear ||||||<html> <a href="http://partsregistry.org/Part:BBa_K613023">BBa_K613023</a></html> ||X|| |
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- | | <html> <a href="http://partsregistry.org/Part:BBa_K613023"> | + | | WT||none||T7||His&GFP||both ||||||<html> <a href="http://partsregistry.org/Part:BBa_K613023"> </a></html>|| || |
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{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} |
Revision as of 18:34, 21 September 2011
muTetRs
In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outlineWhy
How
What
TetR variants | Mutagenesis | Promoter | Cterminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry |
---|---|---|---|---|---|---|---|---|---|
V36F | site directed | SP6 | eGFP | plasmid | worked well | X | BBa_K613013 | ||
V36FW43S | PCR-induced | T7 | His-tag | linear | (ins C-term), ITT tested | BBa_K613014 | |||
E37AW43ST141A | PCR-induced | T7 | His-tag | linear | ITT tested | X | BBa_K613015 | X | X |
P39K | PCR-induced | T7 | His-tag | template | ITT tested, worked | X | BBa_K613016 | X | X |
Y42F | PCR-induced | T7 | His-tag | linear | ITT tested | X | BBa_K613017 | X | X |
Y42FK108E | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613018 | X | X | |
P39QY42M | site directed | SP6 | eGFP | plasmid | worked well | X | BBa_K613019 | ||
P39QY42ML197S | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613020 | |||
P39QY42ML52P | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613021 | |||
E37AP39K | site directed | T7 | His-tag | linear | BBa_K613022 | X | |||
E37AP39QY42F | site directed | T7 | His-tag | linear | BBa_K613023 | X | |||
WT | none | T7 | His&GFP | both |