Team:EPF-Lausanne/Our Project/T7 promoter variants/selection

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(Difference between revisions)
(Results)
(Experimental Setup)
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=== Experimental Setup ===
=== Experimental Setup ===
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To that end, we again set up an experiment involving two flasks but this time each flask contains two types of cells. For one flask, one culture is a co-transformation of a lysis plasmid (C2) and a RFP-containing plasmid and the other is a co-transformation of a negative control plasmid (C11) with a GFP-containing plasmid. In the other flask, the reverse is true: lysis (C2) is with GFP and negative control (C11) is with RFP. This co-culture experiment was prepared by mixing equal amounts of cells from an overnight culture in one big flask. After a 1h culture, we induced lysis with 500 µM IPTG. If you would like to find out more about how IPTG induction tests work, please click [[EPF-Lausanne/Our Project/T7 promoter variants/lysis/iptg|here]].  
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To that end, we again set up an experiment involving two flasks but this time each flask contains two types of cells. For one flask, cell type is a co-transformation of a lysis plasmid (C2) and a RFP-containing plasmid and the other is a co-transformation of a negative control plasmid (C11) with a GFP-containing plasmid. In the other flask, the reverse is true: lysis (C2) is with GFP and negative control (C11) is with RFP. This co-culture experiment was prepared by mixing equal amounts of cells from an overnight culture in one big flask. After a 1h culture, we induced lysis with 500 µM IPTG. If you would like to find out more about how IPTG induction tests work, please click [[EPF-Lausanne/Our Project/T7 promoter variants/lysis/iptg|here]].  
   
   

Revision as of 17:47, 21 September 2011