Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs

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Revision as of 16:44, 21 September 2011

Introduction
Our Project
Tools
- Gibson Assembly
- MITOMI
Notebook
- Protocols
- May
- June
- July
- August
- September
- October
Considerations
- Human Practices
- Safety
Acknowledgements
- Sponsors
- Partners

muTetRs

In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outline

Why

How

What

TetR variants	Method	Promoter	C-terminal	DNA form	Expression	MITOMI vs 1-off	Biobrick	Sequenced	Sent to registry

V36F	site directed mutagenesis	SP6	eGFP	plasmid	worked well	v	BBa_K613013
V36FW43S	PCR-induced mutagenesis	T7	His-tag	linear	(ins C-term), ITT tested		BBa_K613014

E37AW43ST141A	PCR-induced mutagenesis	T7	His-tag	linear	ITT tested	v	BBa_K613015	v	v


P39K	PCR-induced mutagenesis	T7	His-tag	template	ITT tested and worked ok	v	BBa_K613016	v	v

Y42F	PCR-induced mutagenesis	T7	His-tag	linear	ITT tested	v	BBa_K613017	v	v
Y42FK108E	PCR-induced mutagenesis	T7	His-tag	linear	ITT tested		BBa_K613018	v	v

P39QY42M	site directed mutagenesis	SP6	eGFP	plasmid	worked well	v	BBa_K613019
P39QY42ML197S	PCR-induced mutagenesis	T7	His-tag	linear	ITT tested		BBa_K613020
P39QY42ML52P	PCR-induced mutagenesis	T7	His-tag	linear	ITT tested		BBa_K613021

E37AP39K	site directed mutagenesis	T7	His-tag	linear			BBa_K613022	v

E37AP39QY42F	site directed mutagenesis	T7	His-tag	linear			BBa_K613023	v

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