"
Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs
From 2011.igem.org
(Difference between revisions)
(→What) |
(→What) |
||
| Line 9: | Line 9: | ||
| align="center" style="background:#f0f0f0;"|'''TetR variants''' | | align="center" style="background:#f0f0f0;"|'''TetR variants''' | ||
| align="center" style="background:#f0f0f0;"|'''Method''' | | align="center" style="background:#f0f0f0;"|'''Method''' | ||
| - | | align="center" style="background:#f0f0f0;"|'''''' | + | | align="center" style="background:#f0f0f0;"|'''Promoter''' |
| - | | align="center" style="background:#f0f0f0;"|'''''' | + | | align="center" style="background:#f0f0f0;"|'''C-terminal''' |
| - | | align="center" style="background:#f0f0f0;"|''''' | + | | align="center" style="background:#f0f0f0;"|'''DNA form''' |
| align="center" style="background:#f0f0f0;"|'''Expression''' | | align="center" style="background:#f0f0f0;"|'''Expression''' | ||
| align="center" style="background:#f0f0f0;"|'''MITOMI vs 1-off''' | | align="center" style="background:#f0f0f0;"|'''MITOMI vs 1-off''' | ||
| Line 57: | Line 57: | ||
| E37AP39QY42F||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613023 ||v|||| | | E37AP39QY42F||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613023 ||v|||| | ||
|} | |} | ||
| - | |||
{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} | ||
Revision as of 16:34, 21 September 2011
muTetRs
In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outlineWhy
How
What
| TetR variants | Method | Promoter | C-terminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry | in vivo test |
| V36F | site directed mutagenesis | SP6 | eGFP | plasmid | worked well | v | BBa_K613013 | might not bind ATC | ||
| V36FW43S | PCR-induced mutagenesis | T7 | His-tag | linear | (ins C-term), ITT tested | BBa_K613014 | might not bind ATC | |||
| E37AW43ST141A | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | v | BBa_K613015 | v | v | might not bind ATC |
| P39K | PCR-induced mutagenesis | T7 | His-tag | template | ITT tested and worked ok | v | BBa_K613016 | v | v | failed (Gibson?) will be sequenced |
| Y42F | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | v | BBa_K613017 | v | v | Gibson failed |
| Y42FK108E | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | BBa_K613018 | v | v | behaves as WT | |
| P39QY42M | site directed mutagenesis | SP6 | eGFP | plasmid | worked well | v | BBa_K613019 | failed (Gibson?) will be sequenced | ||
| P39QY42ML197S | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | BBa_K613020 | ||||
| P39QY42ML52P | PCR-induced mutagenesis | T7 | His-tag | linear | ITT tested | BBa_K613021 | ||||
| E37AP39K | site directed mutagenesis | T7 | His-tag | linear | BBa_K613022 | v | ||||
| E37AP39QY42F | site directed mutagenesis | T7 | His-tag | linear | BBa_K613023 | v |
