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Team:Paris Liliane Bettencourt/Notebook/2011/07/14/
From 2011.igem.org
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*Put liquid on trash and vortex it | *Put liquid on trash and vortex it | ||
*Protocol of QIAprep Spin Miniprep Kit using a microcentrifuge | *Protocol of QIAprep Spin Miniprep Kit using a microcentrifuge | ||
| + | |||
| + | |||
| + | ==Mathias & Laura== | ||
| + | |||
| + | Reporter BioBricks | ||
| + | |||
| + | ===Miniprep=== | ||
| + | |||
| + | -RFP (m-cherry) E1010 | ||
| + | |||
| + | -Standard Terminator B0015 | ||
| + | |||
| + | -GFP mut3b E0040 | ||
| + | |||
| + | -T7 Terminator J18913 | ||
| + | |||
| + | -pSB1C3 | ||
| + | |||
| + | ===Digestion and gel extraction=== | ||
| + | |||
| + | -RFP (m-cherry) by EcoR1 and Spe1 | ||
| + | |||
| + | -Standard Terminator by EcoR1 and Xba1 | ||
| + | |||
| + | -GFP mut3b by EcoR1 and Spe1 | ||
| + | |||
| + | -T7 Terminator by EcoR1 and Xba1 | ||
| + | |||
| + | -pSB1C3 by EcoR1 and Pst1 | ||
| + | |||
| + | ===Ligation and transformation=== | ||
| + | |||
| + | -RFP & Standard Terminator => K606012 | ||
| + | |||
| + | -GFP mut3b & T7 Terminator => K606014 | ||
| + | |||
| + | -pSB1C3 & RBS => K606061 | ||
Revision as of 15:57, 21 September 2011
==Kevin==
Miniprep of lacO/tetO plasmids :
- Centrifugation of glycerol culturs 4000 RPM - 10 min
- Put liquid on trash and vortex it
- Protocol of QIAprep Spin Miniprep Kit using a microcentrifuge
Contents |
Mathias & Laura
Reporter BioBricks
Miniprep
-RFP (m-cherry) E1010
-Standard Terminator B0015
-GFP mut3b E0040
-T7 Terminator J18913
-pSB1C3
Digestion and gel extraction
-RFP (m-cherry) by EcoR1 and Spe1
-Standard Terminator by EcoR1 and Xba1
-GFP mut3b by EcoR1 and Spe1
-T7 Terminator by EcoR1 and Xba1
-pSB1C3 by EcoR1 and Pst1
Ligation and transformation
-RFP & Standard Terminator => K606012
-GFP mut3b & T7 Terminator => K606014
-pSB1C3 & RBS => K606061
