Team:UPO-Sevilla/Project/Epigenetic Flip Flop/Data Page

From 2011.igem.org

(Difference between revisions)
Line 20: Line 20:
<style type="text/css">
<style type="text/css">
         .dataPage{width:700px; height:475px; background: url(https://static.igem.org/mediawiki/2011/d/d8/UPOSevillaEpigeneticFlipFlop.png);}
         .dataPage{width:700px; height:475px; background: url(https://static.igem.org/mediawiki/2011/d/d8/UPOSevillaEpigeneticFlipFlop.png);}
-
               .tour_Tn7R{
+
               .tour_nmt41X{
-
   border: 3px solid transparent;
+
   border: 3px solid red;
     bottom: 0;
     bottom: 0;
     display: inline-block;
     display: inline-block;
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     position: relative;
     position: relative;
     width: 4.7em;}
     width: 4.7em;}
-
.tour_FRT{    border: 3px solid transparent;
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.tour_TetR{    border: 3px solid red;
     bottom: 0;
     bottom: 0;
     display: inline-block;
     display: inline-block;
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     position: relative;
     position: relative;
     width: 2em}
     width: 2em}
-
.tour_antib{    border: 3px solid transparent;
+
.tour_nmt1{    border: 3px solid red;
     bottom: 0;
     bottom: 0;
     display: inline-block;
     display: inline-block;
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     position: relative;
     position: relative;
     width: 4em}
     width: 4em}
-
         .tour_your_bioBrick{  
+
         .tour_Adh1{  
-
     border: 3px solid transparent;
+
     border: 3px solid red;
     bottom: 0;
     bottom: 0;
     display: inline-block;
     display: inline-block;
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     position: relative;
     position: relative;
     width: 6.5em;}
     width: 6.5em;}
-
   .tour_R6K_origin{  
+
   .tour_Operator{  
-
     border: 3px solid transparent;
+
     border: 3px solid red;
     bottom: 2em;
     bottom: 2em;
     display: inline-block;
     display: inline-block;
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     top: 3em;
     top: 3em;
     width: 6.7em;}
     width: 6.7em;}
-
         .tour_oriT{    border: 3px solid transparent;
+
         .tour_urg1{    border: 3px solid red;
     display: inline-block;
     display: inline-block;
     height: 1em;
     height: 1em;
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     top: 3em;
     top: 3em;
     width: 4em;}
     width: 4em;}
-
.tour_Transposase_action{   
+
.tour_GFP{   
-
     border: 3px solid transparent;
+
     border: 3px solid red;
     display: inline-block;
     display: inline-block;
     height: 2em;
     height: 2em;
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     top: 3em;
     top: 3em;
     width: 4.7em;}
     width: 4.7em;}
-
.tour_Flp_recombinase_action{  
+
.tour_Act1{  
-
     border: 3px solid transparent;
+
     border: 3px solid red;
     display: inline-block;
     display: inline-block;
     height: 4em;
     height: 4em;
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     top: 11em;
     top: 11em;
     width: 6.7em;}
     width: 6.7em;}
-
.tour_final_cartoon{   
+
.tour_KAN{   
-
     border: 3px solid transparent;
+
     border: 3px solid red;
     display: inline-block;
     display: inline-block;
     height: 4em;
     height: 4em;
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                             <div class="dataPage">
                             <div class="dataPage">
-
                               <div class="tour_Tn7R"></div>
+
                               <div class="tour_nmt41X"></div>
-
                               <div class="tour_FRT"></div>
+
                               <div class="tour_TetR"></div>
-
                               <div class="tour_antib"></div>
+
                               <div class="tour_nmt1"></div>
-
                               <div class="tour_your_bioBrick"></div>
+
                               <div class="tour_Adh1"></div>
-
                               <div class="tour_R6K_origin"></div>
+
                               <div class="tour_Operator"></div>
-
                               <div class="tour_oriT"></div>
+
                               <div class="tour_urg1"></div>
-
                               <div class="tour_Transposase_action"></div>
+
                               <div class="tour_GFP"></div>
   
   
-
      <div class="tour_Flp_recombinase_action"></div>
+
      <div class="tour_Act1"></div>
-
      <div class="tour_final_cartoon"></div>
+
      <div class="tour_KAN"></div>
                </div>
                </div>
</div>
</div>
Line 153: Line 153:
var config = [
var config = [
{
{
-
"name" : "tour_Tn7R",
+
"name" : "tour_nmt41X",
"bgcolor" : "white",
"bgcolor" : "white",
"color" : "black",
"color" : "black",
"position" : "T",
"position" : "T",
-
"text" : "Tn7R & Tn7L: ends of Tn7 transposon, required for transposition. They are recognized by the transposase machinery, which will transpose any sequence located between these two motifs.",
+
"text" : "nmt41X (no message in thiamine) promoter: Repressible promoter by thiamine.",
"time" : 5000
"time" : 5000
},
},
{
{
-
"name" : "tour_FRT",
+
"name" : "tour_TetR",
"bgcolor" : "white",
"bgcolor" : "white",
"color" : "black",
"color" : "black",
-
"text" : "FRT: Flp recombinase target sites, required for excision of the resistance cassette.",
+
"text" : "TetR-SP (silencing protein). Composed of  tetracycline repressor and Swi6, this fusion protein is responsible for binding to tetO sequences, flanking reporter marker, and generating a nucleated structure of silencing proteins around  P urg1 promoter. ",
"position" : "T",
"position" : "T",
"time" : 5000
"time" : 5000
},
},
{
{
-
"name" : "tour_antib",
+
"name" : "tour_nmt1",
"bgcolor" : "white",
"bgcolor" : "white",
"color" : "black",
"color" : "black",
-
"text" : "Antibiotic resistance cassette for selection of strains bearing transposon insertions. After that it can be removed by site-specific recombination at the flanking FRT sites.",
+
"text" : "nmt1 Terminator. Transcription terminator of nmt1.",
"position" : "T",
"position" : "T",
"time" : 5000
"time" : 5000
},
},
{
{
-
"name" : "tour_your_bioBrick",
+
"name" : "tour_Adh1",
"bgcolor" : "white",
"bgcolor" : "white",
"color" : "black",
"color" : "black",
-
"text" : "Your BioBrick: You can use the BioBrick cloning site to clone the part(s) you wish to insert at the microbial genome.",
+
"text" : "Adh1 Terminator. Transcription terminator of Adh1 gene.",
"position" : "T",
"position" : "T",
"time" : 5000
"time" : 5000
},
},
{
{
-
"name" : "tour_R6K_origin",
+
"name" : "tour_Operator",
"bgcolor" : "white",
"bgcolor" : "white",
"color" : "black",
"color" : "black",
"position" : "T",
"position" : "T",
-
"text" : "R6K origin: replication origin only active in pir+ E. coli strains.",
+
"text" : "Operator sites of tetR (tetO). The binding of  fusion silencing proteins to this site give rise to compaction of reporter module and silencing of urg1 promoter.",
"time" : 5000
"time" : 5000
},
},
{
{
-
"name" : "tour_oriT",
+
"name" : "tour_urg1",
"bgcolor" : "white",
"bgcolor" : "white",
"color" : "black",
"color" : "black",
-
"text" : "oriT: transference origin for conjugation purposes.",
+
"text" : "urg1 promoter. uracil-inducible urg1 promoter, that allows expression of reporter GFP . (Watt et al, 2008) ",
"position" : "T",
"position" : "T",
"time" : 5000
"time" : 5000
},
},
{
{
-
"name" : "tour_Transposase_action",
+
"name" : "tour_GFP",
"bgcolor" : "white",
"bgcolor" : "white",
"color" : "black",
"color" : "black",
-
"text" : "Transposase action: The transient expression of the transposase complex causes single site-specific genome integration at the attTn7 site.",
+
"text" : "GFP. Marker module used to measure the efficiency of the designed syetem.",
"position" : "T",
"position" : "T",
"time" : 5000
"time" : 5000
},
},
{
{
-
"name" : "tour_Flp_recombinase_action",
+
"name" : "tour_Act1",
"bgcolor" : "white",
"bgcolor" : "white",
"color" : "black",
"color" : "black",
-
"text" : "Flp recombinase action: The transient expression of Flp recombinase or Flipase induces recombination between FRT sites, removing the resistance cassette between them.",
+
"text" : "Act1 Terminator. Transcription terminator of act1 gene.",
"position" : "T",
"position" : "T",
"time" : 5000
"time" : 5000
},
},
                     {
                     {
-
"name" : "tour_final_cartoon",
+
"name" : "tour_KAN",
"bgcolor" : "white",
"bgcolor" : "white",
"color" : "black",
"color" : "black",
-
"text" : "Finally you get your BioBrick into of a new strain of bacterium without plasmid limitations or antibiotic resistance disadvantages.",
+
"text" : "KAN. Marker cassette for selection after positive integration of reporter module in S. pombe genome.",
"position" : "B",
"position" : "B",
"time" : 5000
"time" : 5000

Revision as of 10:57, 21 September 2011

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Data Page

Retrieved from "http://2011.igem.org/Team:UPO-Sevilla/Project/Epigenetic_Flip_Flop/Data_Page"