Team:UT-Tokyo/Data/Method

From 2011.igem.org

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Revision as of 10:47, 21 September 2011

Reagents

  • LB broth
reagents final conc. amount
Bacto trypton 1% 1g
NaCl 0.5% 0.5g
Yeast extracs 0.5% 0.5g
MilliQ - 100ml
Total 100ml


  • SOB broth
reagents final conc. amount
Bacto trypton 2% 20g
NaCl 0.05% 0.5g
Yeast extracs 0.5% 5g
KCl (250mM) 2.5mM 10ml
MilliQ - 1000ml
Total 1L

Before use, add 10ml Mg sol.

Mg sol.

reagents final conc. amount
MgCl2(H2O)6 1M 20.33g
MgSO4(H2O)7 1M 24.648g
MilliQ - 100ml
Total 100ml


  • M9
  • 50× TAE
reagents final conc. amount
Tris 2M 242g
CH3COOH 1M 57.1mL
EDTA (0.5M, pH=8.0) 0.05M 100ml
MilliQ - 1000ml
Total 1L
  • TB
reagents final conc. amount
KOH 500mM sol. 250MM 242g
PIPES 500mM sol. 10mM 2ml
CaCl2 750mM sol. 15mM 2ml
KCl 2.5M sol. 250mM 10ml
MnCl2 550mM sol. 55mM 10ml
MilliQ - 100ml
Total 100ml

Cultures

Extraction of DNA from cells

Amplification of DNA

Analysis of DNA

Assays

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