Team:UT-Tokyo/Data/Method

From 2011.igem.org

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==Reagents==
==Reagents==
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<div style="float:left">
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<div class="float-left">
*LB broth
*LB broth
{|
{|
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Before use, add 10ml Mg sol.
Before use, add 10ml Mg sol.
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;:Mg sol.
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-
::{|
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Mg sol.
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|MgCl<span style="vertical-align: sub;">2</span>
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{|
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|500mM
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!align="left" style="width:200px"|reagents
 +
!align="right" style="width:80px"|final conc.
 +
!align="right" style="width:80px"|amount
|-
|-
-
|MgSO<span style="vertical-align: sub;">4</span>
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|MgCl<span class="sub">2</span>(H<span class="sub">2</span>O)<span class="sub">6</span>
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|500mM
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|align="right"|1M
 +
|align="right"|20.33g
 +
|-
 +
|MgSO<span class="sub">4</span>(H<span class="sub">2</span>O)<span class="sub">7</span>
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|align="right"|1M
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|align="right"|24.648g
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|-
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|style="border-bottom: 2px solid"|MilliQ
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|style="text-align:center; border-bottom: 2px solid"|-
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|style="text-align:right; border-bottom: 2px solid"|100ml
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|-
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|colspan="2"|Total
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|style="text-align:right"|100ml
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|align="right"|242g
|align="right"|242g
|-
|-
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|CH<span style="vertical-align: sub;">3</span>COOH
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|CH<span class="sub">3</span>COOH
|align="right"|1M
|align="right"|1M
|align="right"|57.1mL
|align="right"|57.1mL
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|-
|-
|KOH 500mM sol.
|KOH 500mM sol.
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|align="right"|250mMM
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|align="right"|250MM
|align="right"|242g
|align="right"|242g
|-
|-
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|align="right"|2ml
|align="right"|2ml
|-
|-
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|CaCl<span style="vertical-align: sub;">2</span> 750mM sol.  
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|CaCl<span class="sub">2</span> 750mM sol.  
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|align="right"15mM
+
|align="right"|15mM
|align="right"|2ml
|align="right"|2ml
|-
|-
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|align="right"|10ml
|align="right"|10ml
|-
|-
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|MnCl<span style="vertical-align: sub;">2</span> 550mM sol.
+
|MnCl<span class="sub">2</span> 550mM sol.
|align="right"|55mM
|align="right"|55mM
|align="right"|10ml
|align="right"|10ml

Revision as of 10:18, 21 September 2011

Reagents

  • LB broth
reagents final conc. amount
Bacto trypton 1% 1g
NaCl 0.5% 0.5g
Yeast extracs 0.5% 0.5g
MilliQ - 100ml
Total 100ml


  • SOB broth
reagents final conc. amount
Bacto trypton 2% 20g
NaCl 0.05% 0.5g
Yeast extracs 0.5% 5g
KCl (250mM) 2.5mM 10ml
MilliQ - 1000ml
Total 1L

Before use, add 10ml Mg sol.

Mg sol.

reagents final conc. amount
MgCl2(H2O)6 1M 20.33g
MgSO4(H2O)7 1M 24.648g
MilliQ - 100ml
Total 100ml


  • M9
  • 50× TAE
reagents final conc. amount
Tris 2M 242g
CH3COOH 1M 57.1mL
EDTA (0.5M, pH=8.0) 0.05M 100ml
MilliQ - 1000ml
Total 1L
  • TB
reagents final conc. amount
KOH 500mM sol. 250MM 242g
PIPES 500mM sol. 10mM 2ml
CaCl2 750mM sol. 15mM 2ml
KCl 2.5M sol. 250mM 10ml
MnCl2 550mM sol. 55mM 10ml
MilliQ - 100ml
Total 100ml

Cultures

Extraction of DNA from cells

Amplification of DNA

Analysis of DNA

Assays

Retrieved from "http://2011.igem.org/Team:UT-Tokyo/Data/Method"