Team:EPF-Lausanne/Tools/Microfluidics/HowTo1
From 2011.igem.org
Line 14: | Line 14: | ||
<embed type="application/x-shockwave-flash" src="https://picasaweb.google.com/s/c/bin/slideshow.swf" width="600" height="400" flashvars="host=picasaweb.google.com&hl=en_US&feat=flashalbum&RGB=0x000000&feed=https%3A%2F%2Fpicasaweb.google.com%2Fdata%2Ffeed%2Fapi%2Fuser%2F114670117111403230486%2Falbumid%2F5654596587940840561%3Falt%3Drss%26kind%3Dphoto%26hl%3Den_US" pluginspage="http://www.macromedia.com/go/getflashplayer"></embed> | <embed type="application/x-shockwave-flash" src="https://picasaweb.google.com/s/c/bin/slideshow.swf" width="600" height="400" flashvars="host=picasaweb.google.com&hl=en_US&feat=flashalbum&RGB=0x000000&feed=https%3A%2F%2Fpicasaweb.google.com%2Fdata%2Ffeed%2Fapi%2Fuser%2F114670117111403230486%2Falbumid%2F5654596587940840561%3Falt%3Drss%26kind%3Dphoto%26hl%3Den_US" pluginspage="http://www.macromedia.com/go/getflashplayer"></embed> | ||
</html> | </html> | ||
+ | |||
+ | <p> 1 Place molds into a TMCS vapor chamber</p> | ||
+ | <p></p> | ||
+ | <p> 2a) Control layer mixture: 20g Base + 4g Curing agent </p> | ||
+ | <p> 2b) Mix for 1 minute, degas for 2 minutes (standard protocol)</p> | ||
+ | <p> 2c) Pour onto control layer mold and place mold in vacuum chamber for at least 20min</p> | ||
+ | <p></p> | ||
+ | <p> 3a) Flow layer mixture: 20g Base + 1g Curing agent</p> | ||
+ | <p> 3b) Mix for 1 minute and degas for 2 minutes (standard protocol)</p> | ||
+ | <p> 3c) Spin coat onto flow layer at 2200-2400rpm for 35secs</p> | ||
+ | <p></p> | ||
+ | <p> 4 Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some )</p> | ||
+ | <p></p> | ||
+ | <p> 5 Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!)</p> | ||
+ | <p></p> | ||
+ | <p> 6a) Remover wafers both layers from the oven, cut out control layer </p> | ||
+ | <p> 6b) Punch holes </p> | ||
+ | <p> 6c) Align control to the flow layer</p> | ||
+ | <p></p> | ||
+ | <p> 7 Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing time)</p> | ||
+ | <p></p> | ||
+ | <p> 8a) Remove devices from oven</p> | ||
+ | <p> 8b) Cut them out </p> | ||
+ | <p> 8c) Punch flow layer holes and cut the edges through the flow layer</p> | ||
+ | |||
+ | |||
{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} |
Revision as of 00:21, 21 September 2011
Microfluidics how-to part I: making chips
Microfluidics Main | How-To Part I | How-To Part II | TamagotchipTODO: outline wafer fabrication and chip fab. Or just refer to Stanford chip ordering service.
SU8 wafer fabrication (a.k.a. "Dress as an astronaut day"):
MITOMI Chip fabrication:
1 Place molds into a TMCS vapor chamber
2a) Control layer mixture: 20g Base + 4g Curing agent
2b) Mix for 1 minute, degas for 2 minutes (standard protocol)
2c) Pour onto control layer mold and place mold in vacuum chamber for at least 20min
3a) Flow layer mixture: 20g Base + 1g Curing agent
3b) Mix for 1 minute and degas for 2 minutes (standard protocol)
3c) Spin coat onto flow layer at 2200-2400rpm for 35secs
4 Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some )
5 Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!)
6a) Remover wafers both layers from the oven, cut out control layer
6b) Punch holes
6c) Align control to the flow layer
7 Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing time)
8a) Remove devices from oven
8b) Cut them out
8c) Punch flow layer holes and cut the edges through the flow layer