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Revision as of 22:20, 20 September 2011 (view source) Pegasus1504 (Talk | contribs) ← Older edit		Revision as of 22:21, 20 September 2011 (view source) Pegasus1504 (Talk | contribs) (→Rsn2(non-His-tagged)) Newer edit →
Line 27:		Line 27:
-	TAATAA: introduced double stop codon	+	TAATAA: introduced double stop codon.
		+
	Lowercase: biobrick ends on primers.		Lowercase: biobrick ends on primers.
-	No illegal restriction sites.
		+	No illegal restriction sites.
	== Protein Sequence ==		== Protein Sequence ==

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Revision as of 22:21, 20 September 2011

Rsn2(His-tagged)

Nucleotide Sequence:

gtgtgtgaattcgcggccgcttctagagAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCC GCGCGGCAGCCATATGTTAATATTAGATGGGGACCTACTAAAGGACAAGTTAAAGTTACCGGTCATTGACAATCTGTTTGGTAAAGAACTC CTTGACAAGTTTCAAGATGATATTAAGGACAAATATGGGGTGGACACCAAAGACCTTAAGATCCTCAAGACATCAGAAGATAAAAGGTTTT ACTATGTGTCGGTCGATGCTGGAGATGGCGAGAAATGTAAATTCAAGATTAGAAAAGATGTCGATGTTCCAAAGATGGTCGGACGCAAATG TAGGAAGGACGATGATGATGATGATGG(ttac)TAATAAtactagtagcggccgctgcagacacac

TAATAA: introduced double stop codon

Lowercase: biobrick ends on primers. No illegal restriction sites.

Rsn2(non-His-tagged)

Nucleotide Sequence:

gtgtgtgaattcgcggccgcttctagagAGGAGGATTACAAAATGTTAATATTAGATGGGGACCTACTAAAGGACCCATCATCATCATCAT CACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGTTAATATTAGATGGGGACCTACTAAAGGACAAGTTAAAGTTACCGGTCATTGACA ATCTGTTTGGTAAAGAACTCCTTGACAAGTTTCAAGATGATATTAAGGACAAATATGGGGTGGACACCAAAGACCTTAAGATCCTCAAGAC ATCAGAAGATAAAAGGTTTTACTATGTGTCGGTCGATGCTGGAGATGGCGAGAAATGTAAATTCAAGATTAGAAAAGATGTCGATGTTCCA AAGATGGTCGGACGCAAATGTAGGAAGGACGATGATGATGATGATGGTAATAAtactagtagcggccgctgcagacacac

TAATAA: introduced double stop codon.

Lowercase: biobrick ends on primers.

No illegal restriction sites.

Protein Sequence

117 Amino Acids:

MGSSHHHHHH SSGLVPR↓GSH MLILDGDLLK DKLKLPVIDN LFGKELLDKF QDDIKDKYGV DTKDLKILKT SEDKRFYYVS VDAGDGEKCK FKIRKDVDVP KMVGRKCRKD DDDDDGY