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From 2011.igem.org

Rsn2(His-tagged)

Nucleotide Sequence:

gtgtgtgaattcgcggccgcttctagagAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCC GCGCGGCAGCCATATGTTAATATTAGATGGGGACCTACTAAAGGACAAGTTAAAGTTACCGGTCATTGACAATCTGTTTGGTAAAGAACTC CTTGACAAGTTTCAAGATGATATTAAGGACAAATATGGGGTGGACACCAAAGACCTTAAGATCCTCAAGACATCAGAAGATAAAAGGTTTT ACTATGTGTCGGTCGATGCTGGAGATGGCGAGAAATGTAAATTCAAGATTAGAAAAGATGTCGATGTTCCAAAGATGGTCGGACGCAAATG TAGGAAGGACGATGATGATGATGATGG(ttac)TAATAAtactagtagcggccgctgcagacacac

TAATAA: introduced double stop codon.

Lowercase: biobrick ends on primers.

No illegal restriction sites.

Rsn2(non-His-tagged)

Nucleotide Sequence:

gtgtgtgaattcgcggccgcttctagagAGGAGGATTACAAAATGTTAATATTAGATGGGGACCTACTAAAGGACCCATCATCATCATCAT CACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGTTAATATTAGATGGGGACCTACTAAAGGACAAGTTAAAGTTACCGGTCATTGACA ATCTGTTTGGTAAAGAACTCCTTGACAAGTTTCAAGATGATATTAAGGACAAATATGGGGTGGACACCAAAGACCTTAAGATCCTCAAGAC ATCAGAAGATAAAAGGTTTTACTATGTGTCGGTCGATGCTGGAGATGGCGAGAAATGTAAATTCAAGATTAGAAAAGATGTCGATGTTCCA AAGATGGTCGGACGCAAATGTAGGAAGGACGATGATGATGATGATGGTAATAAtactagtagcggccgctgcagacacac

TAATAA: introduced double stop codon.

Lowercase: biobrick ends on primers.

No illegal restriction sites.

Protein Sequence

117 Amino Acids:

MGSSHHHHHH SSGLVPR↓GSH MLILDGDLLK DKLKLPVIDN LFGKELLDKF QDDIKDKYGV DTKDLKILKT SEDKRFYYVS VDAGDGEKCK FKIRKDVDVP KMVGRKCRKD DDDDDGY