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Team:Glasgow/2011.igem.org/Team:Glasgow/Ranaspumin2 Sequence
From 2011.igem.org
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| + | == Rsn2(His-tagged) == | ||
| + | |||
| + | Nucleotide Sequence: | ||
gtgtgtgaattcgcggccgcttctagagAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCC | gtgtgtgaattcgcggccgcttctagagAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCC | ||
| Line 15: | Line 17: | ||
| + | == Rsn2(non-His-tagged) == | ||
| - | + | Nucleotide Sequence: | |
gtgtgtgaattcgcggccgcttctagagAGGAGGATTACAAAATGTTAATATTAGATGGGGACCTACTAAAGGACCCATCATCATCATCAT | gtgtgtgaattcgcggccgcttctagagAGGAGGATTACAAAATGTTAATATTAGATGGGGACCTACTAAAGGACCCATCATCATCATCAT | ||
| Line 30: | Line 33: | ||
| - | + | == Protein Sequence == | |
| + | |||
| + | 117 Amino Acids: | ||
MGSSHHHHHH SSGLVPR↓GSH MLILDGDLLK DKLKLPVIDN LFGKELLDKF QDDIKDKYGV DTKDLKILKT SEDKRFYYVS | MGSSHHHHHH SSGLVPR↓GSH MLILDGDLLK DKLKLPVIDN LFGKELLDKF QDDIKDKYGV DTKDLKILKT SEDKRFYYVS | ||
VDAGDGEKCK FKIRKDVDVP KMVGRKCRKD DDDDDGY | VDAGDGEKCK FKIRKDVDVP KMVGRKCRKD DDDDDGY | ||
Revision as of 22:18, 20 September 2011
Rsn2(His-tagged)
Nucleotide Sequence:
gtgtgtgaattcgcggccgcttctagagAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCC GCGCGGCAGCCATATGTTAATATTAGATGGGGACCTACTAAAGGACAAGTTAAAGTTACCGGTCATTGACAATCTGTTTGGTAAAGAACTC CTTGACAAGTTTCAAGATGATATTAAGGACAAATATGGGGTGGACACCAAAGACCTTAAGATCCTCAAGACATCAGAAGATAAAAGGTTTT ACTATGTGTCGGTCGATGCTGGAGATGGCGAGAAATGTAAATTCAAGATTAGAAAAGATGTCGATGTTCCAAAGATGGTCGGACGCAAATG TAGGAAGGACGATGATGATGATGATGG(ttac)TAATAAtactagtagcggccgctgcagacacac
TAATAA: introduced double stop codon
Lowercase: biobrick ends on primers. No illegal restriction sites.
Rsn2(non-His-tagged)
Nucleotide Sequence:
gtgtgtgaattcgcggccgcttctagagAGGAGGATTACAAAATGTTAATATTAGATGGGGACCTACTAAAGGACCCATCATCATCATCAT CACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGTTAATATTAGATGGGGACCTACTAAAGGACAAGTTAAAGTTACCGGTCATTGACA ATCTGTTTGGTAAAGAACTCCTTGACAAGTTTCAAGATGATATTAAGGACAAATATGGGGTGGACACCAAAGACCTTAAGATCCTCAAGAC ATCAGAAGATAAAAGGTTTTACTATGTGTCGGTCGATGCTGGAGATGGCGAGAAATGTAAATTCAAGATTAGAAAAGATGTCGATGTTCCA AAGATGGTCGGACGCAAATGTAGGAAGGACGATGATGATGATGATGGTAATAAtactagtagcggccgctgcagacacac
Red primers
Lowercase: biobrick ends on primers.
No illegal restriction sites.
Protein Sequence
117 Amino Acids:
MGSSHHHHHH SSGLVPR↓GSH MLILDGDLLK DKLKLPVIDN LFGKELLDKF QDDIKDKYGV DTKDLKILKT SEDKRFYYVS VDAGDGEKCK FKIRKDVDVP KMVGRKCRKD DDDDDGY
