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Team:Glasgow/2011.igem.org/Team:Glasgow/Ranaspumin2 Sequence
From 2011.igem.org
(Created page with "Rsn2(His-tagged) Nucleotide Sequence: gtgtgtgaattcgcggccgcttctagagAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCC GCGCGGCAGCCATATGTTAATATTAGATGGGGACCTACTAAAGGAC...")
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Revision as of 22:15, 20 September 2011
Rsn2(His-tagged) Nucleotide Sequence:
gtgtgtgaattcgcggccgcttctagagAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCC
GCGCGGCAGCCATATGTTAATATTAGATGGGGACCTACTAAAGGACAAGTTAAAGTTACCGGTCATTGACAATCTGTTTGGTAAAGAACTC
CTTGACAAGTTTCAAGATGATATTAAGGACAAATATGGGGTGGACACCAAAGACCTTAAGATCCTCAAGACATCAGAAGATAAAAGGTTTT
ACTATGTGTCGGTCGATGCTGGAGATGGCGAGAAATGTAAATTCAAGATTAGAAAAGATGTCGATGTTCCAAAGATGGTCGGACGCAAATG
TAGGAAGGACGATGATGATGATGATGG(ttac)TAATAAtactagtagcggccgctgcagacacac
TAATAA: introduced double stop codon
Lowercase: biobrick ends on primers. No illegal restriction sites.
Rsn2(non-His-tagged) Nucleotide Sequence:
gtgtgtgaattcgcggccgcttctagagAGGAGGATTACAAAATGTTAATATTAGATGGGGACCTACTAAAGGACCCATCATCATCATCAT CACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGTTAATATTAGATGGGGACCTACTAAAGGACAAGTTAAAGTTACCGGTCATTGACA ATCTGTTTGGTAAAGAACTCCTTGACAAGTTTCAAGATGATATTAAGGACAAATATGGGGTGGACACCAAAGACCTTAAGATCCTCAAGAC ATCAGAAGATAAAAGGTTTTACTATGTGTCGGTCGATGCTGGAGATGGCGAGAAATGTAAATTCAAGATTAGAAAAGATGTCGATGTTCCA AAGATGGTCGGACGCAAATGTAGGAAGGACGATGATGATGATGATGGTAATAAtactagtagcggccgctgcagacacac
Red primers
Lowercase: biobrick ends on primers.
No illegal restriction sites.
ProductSequence: 117 Amino Acids:
MGSSHHHHHH SSGLVPR↓GSH MLILDGDLLK DKLKLPVIDN LFGKELLDKF QDDIKDKYGV DTKDLKILKT SEDKRFYYVS VDAGDGEKCK FKIRKDVDVP KMVGRKCRKD DDDDDGY
