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Team:Groningen/project characterisation mu
From 2011.igem.org
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{| class="wikitable" | {| class="wikitable" | ||
| - | ! colspan=" | + | ! colspan="2" | cI autoinducing loop (BBa_K00) after 4 hours of incubation |
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| [[File:ci1ui.png]] | | [[File:ci1ui.png]] | ||
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| Non-Fluorescent|| 34424 || 68 || 5974 | | Non-Fluorescent|| 34424 || 68 || 5974 | ||
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| - | |||
|- | |- | ||
| + | | colspan=2 | Two populations are visible blabla | ||
| + | |-0 | ||
| Cel 7 || Cel 8 || Cel 9 | | Cel 7 || Cel 8 || Cel 9 | ||
|} | |} | ||
{{FooterGroningen2011}} | {{FooterGroningen2011}} | ||
Revision as of 21:51, 20 September 2011
Memory Units
cI Autoinducing Loops
The autoinducing loop driven by cI will function as our first memory unit. We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. To estimate the rate in which single cells are activated by too much leakage of cI protein we measured the fluorescence in uninduced cells with a flow cytometer. To measure the activation we used a construct with the PRM promoter producing GFP.
| cI autoinducing loop (BBa_K00) after 4 hours of incubation | |||||||||||||||||
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| Two populations are visible blabla | |||||||||||||||||
| Cel 7 | Cel 8 | Cel 9 | |||||||||||||||
