Team:Groningen/project characterisation mu

From 2011.igem.org

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==cI Autoinducing Loops==
==cI Autoinducing Loops==
The autoinducing loop driven by cI will function as our first memory unit.  
The autoinducing loop driven by cI will function as our first memory unit.  
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We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. To estimate the rate in which single cells are activated by too much leakage of cI protein we meassured the fluorescence in uninduced cells with a flow cytometer.
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We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. To estimate the rate in which single cells are activated by too much leakage of cI protein we measured the fluorescence in uninduced cells with a flow cytometer. To measure the activation we used a construct with the PRM promoter producing GFP.  
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{| class="wikitable"
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! colspan="3" | cI autoinducing loop (BBa_K00) after 4 hours of incubation
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|-
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| File:cI4.png
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|
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{| class="wikitable"
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! 
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! Number
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! % of total
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! Mean fluorescence
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|-
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| Visible
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| 50000
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| 100
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| ---
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|-
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| Fluorescent || 9180 || 18,36 || 11419
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|-
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| Non-Fluorescent|| 34424 || 68 || 5974
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|}
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| Cel 6
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|-
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| Cel 7 || Cel 8 || Cel 9
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|}
{{FooterGroningen2011}}
{{FooterGroningen2011}}

Revision as of 21:40, 20 September 2011


Memory Units

cI Autoinducing Loops

The autoinducing loop driven by cI will function as our first memory unit. We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. To estimate the rate in which single cells are activated by too much leakage of cI protein we measured the fluorescence in uninduced cells with a flow cytometer. To measure the activation we used a construct with the PRM promoter producing GFP.

cI autoinducing loop (BBa_K00) after 4 hours of incubation
File:cI4.png
Number  % of total Mean fluorescence
Visible 50000 100 ---
Fluorescent 9180 18,36 11419
Non-Fluorescent 34424 68 5974
Cel 6
Cel 7 Cel 8 Cel 9




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