Team:Paris Bettencourt/Experiments/T7 diffusion

• Home
• Presentation
  • Overview
  • Potential applications
  • Collaborations
  • The team
  • Acknowledgements →
    • Host laboratory
    • Sponsors
• Designs
  • Designs overview & concepts
  • Designs list
• Modeling
  • General overview
  • Modeling results for our designs →
    • The basics
    • Our assumptions
    • T7 polymerase diffusion
    • tRNA diffusion
    • ComK/ComS system
    • Parameters
  • Results of our passive diffusion model
  • Toward assisted diffusion
• Data
• Results
  • Achievements
  • Methodologies →
    • Microscopy
    • Micro-fluidic
    • Integration in B.subtilis
    • Kinetics
  • Parts list
  • Notebook
• Human practice & Safety
  • Human practice
  • Safety
  • Brainstorming
  • Bonus track

Characterization of the T7 signal amplification leakage in E. Coli

We characterized T7 autoloop in E. Coli, when hosted in the plasmid pSB1C3 (the K606036 part). The idea was to see if the system was leaky inside a synthetic biology plasmid, that is to say, a plasmid holding 4 terminators before any construct.

We found out that there is a leakage, but it is small. The cells in which the positive feed back look is activated stop deviding and glow with a very strong signal. Here are the images commented

T7 autoloop RFP+ Control / E-coli at 37°C

E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	-Coli at 37°C (rfp image)

TEXT

T7 autoloop full no terminator / E-coli at 37°C

E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	-Coli at 37°C (rfp image)

T7 autoloop full with terminator / E-coli at 37°C

E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)	E-Coli at 37°C (gfp image)	-Coli at 37°C (rfp image)

• Recent changes
• What links here
• Related changes
• Upload file
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers