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Team:Groningen/project characterisation promoters pbad
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(Created page with "{{HeaderGroningen2011}} In our system and measurements we have been using the pBAD/AraC promoter ([http://partsregistry.org/Part:BBa_I0500 BBa_I0500]). This promoter is functioni...")
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(Created page with "{{HeaderGroningen2011}} In our system and measurements we have been using the pBAD/AraC promoter ([http://partsregistry.org/Part:BBa_I0500 BBa_I0500]). This promoter is functioni...")
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Revision as of 16:53, 20 September 2011
In our system and measurements we have been using the pBAD/AraC promoter ([http://partsregistry.org/Part:BBa_I0500 BBa_I0500]). This promoter is functioning in our circuit as a jammer for the LasR autoinducing loop. It is also used to induce the cI autoinducing loops during the measurements. That is why, in our opinion, characterizing this promoter in a way that would show us the activity rate not only depending on concentration of arabinose, but also in a dynamic way - over time - is crucial.
The measurements were done as described in protocol.
Experiment 1b. Cells carrying a pSB1C3 plasmid with pBAD/AraC-GFP-DT construct, induced at time=0h.
Experiment 1a. Wild type cells, induced at time=0h.
Experiment 2b. Cells carrying a pSB1C3 plasmid with pBAD/AraC-GFP-DT construct, induced at time=0h.
Experiment 2a. Wild type cells, induced at time=0h.
Below you will find four figures representing the results of our measurements.
