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Team:Groningen/project notebook/14 September 2011
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measurement over time with flow cytometry (0 - 8 h) | measurement over time with flow cytometry (0 - 8 h) | ||
*measurement | *measurement | ||
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| + | '''Joyce''' | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> Plasmid prep of other colonies of PBAD-cI-LVA-DT and PBAD-LasR-LVA-DT | ||
| + | <br> After prep checking on the nanodrop: concentration was low, because it was eluted in higher volume. | ||
| + | <br> Samples were put in the vaquum centrifuge at 60 °C for 20 minutes. | ||
| + | <br> Samples were measured again with nanodrop: plots looked nicer and DNA concentration was higher, up to 60 μg/μl | ||
| + | <br> | ||
| + | <br> Transformation of yesterday with all the plates did go well, 25 of the 28 samples show colonies on the plate | ||
| + | <br> | ||
| + | <br> Prepared 100 tubes with LB+ Chloramphenicol and inoculated them, since we need 4 of each sample for plasmid prepping tomorrow | ||
| + | <br> | ||
| + | <br> 15:15: hurrying, because tonight the choir song will be recorded with professional recording stuff! | ||
| + | <br> Getting software for adapting the record and the film synchronized, finished at 11:00 | ||
| + | <br> | ||
| + | |||
{{FooterGroningen2011}} | {{FooterGroningen2011}} | ||
Latest revision as of 15:26, 20 September 2011
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Olesja
- measurement of leakage of LasR autoinducing loops (with 100 nM HSL)
measurement over time with flow cytometry (0 - 8 h)
- measurement
Joyce
Plasmid prep of other colonies of PBAD-cI-LVA-DT and PBAD-LasR-LVA-DT
After prep checking on the nanodrop: concentration was low, because it was eluted in higher volume.
Samples were put in the vaquum centrifuge at 60 °C for 20 minutes.
Samples were measured again with nanodrop: plots looked nicer and DNA concentration was higher, up to 60 μg/μl
Transformation of yesterday with all the plates did go well, 25 of the 28 samples show colonies on the plate
Prepared 100 tubes with LB+ Chloramphenicol and inoculated them, since we need 4 of each sample for plasmid prepping tomorrow
15:15: hurrying, because tonight the choir song will be recorded with professional recording stuff!
Getting software for adapting the record and the film synchronized, finished at 11:00
