Team:Osaka/week8
From 2011.igem.org
(Difference between revisions)
Line 150: | Line 150: | ||
|- | |- | ||
|wild type-5||5.0|| | |wild type-5||5.0|| | ||
+ | |} | ||
+ | # Plating (cell survival) | ||
+ | {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | ||
+ | |+Table 9 | ||
+ | !Name!!OD600!!UV(mJ/cm^2) | ||
+ | |- | ||
+ | |PprI-1||0.0109||0 | ||
+ | |- | ||
+ | |PprI-2||0.0109||0.5 | ||
+ | |- | ||
+ | |PprI-3||0.0109||1.0 | ||
+ | |- | ||
+ | |PprI-4||0.0109||2.0 | ||
+ | |- | ||
+ | |PprI-5||0.0109||5.0 | ||
+ | |- | ||
+ | |PprA-1||0.0418||0 | ||
+ | |- | ||
+ | |PprA-2||0.0418||0.5 | ||
+ | |- | ||
+ | |PprA-3||0.0418||1.0 | ||
+ | |- | ||
+ | |PprA-4||0.0418||2.0 | ||
+ | |- | ||
+ | |PprA-5||0.0418||5.0 | ||
+ | |- | ||
+ | |PprM-1||0.0454||0 | ||
+ | |- | ||
+ | |PprM-2||0.0454||0.5 | ||
+ | |- | ||
+ | |PprM-3||0.0454||1.0 | ||
+ | |- | ||
+ | |PprM-4||0.0454||2.0 | ||
+ | |- | ||
+ | |PprM-5||0.0454||5.0 | ||
+ | |- | ||
+ | |RecA-1||0.0114||0 | ||
+ | |- | ||
+ | |RecA-2||0.0114||0.5 | ||
+ | |- | ||
+ | |RecA-3||0.0114||1.0 | ||
+ | |- | ||
+ | |RecA-4||0.0114||2.0 | ||
+ | |- | ||
+ | |RecA-5||0.0114||5.0 | ||
+ | |- | ||
+ | |empty vector-1||0.0515||0 | ||
+ | |- | ||
+ | |empty vector-2||0.0515||0.5 | ||
+ | |- | ||
+ | |empty vector-3||0.0515||1.0 | ||
+ | |- | ||
+ | |empty vector-4||0.0515||2.0 | ||
+ | |- | ||
+ | |empty vector-5||0.0515||5.0 | ||
+ | |- | ||
+ | |wild type-1||0.0546||0 | ||
+ | |- | ||
+ | |wild type-2||0.0546||0.5 | ||
+ | |- | ||
+ | |wild type-3||0.0546||1.0 | ||
+ | |- | ||
+ | |wild type-4||0.0546||2.0 | ||
+ | |- | ||
+ | |wild type-5||0.0546||5.0 | ||
|} | |} | ||
+ | |||
Revision as of 13:31, 20 September 2011
Contents |
September 18(Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- To liquid culture:03,105,106,107,108,109,110,111,112
September 19(Mon)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Ligation
- 113(T):105(upstream)+110(downstream)+1-7A(vector)
- 114(T):111(upstream)+112(downstream)+1-7A(vector)
- Transformation of ligation products
- Plating (cell survival)
Name | OD600 | UV(mJ/cm^2) |
---|---|---|
PprI-1 | 0.0109 | 0 |
PprI-2 | 0.0109 | 0.5 |
PprI-3 | 0.0109 | 1.0 |
PprI-4 | 0.0109 | 2.0 |
PprI-5 | 0.0109 | 5.0 |
PprA-1 | 0.0418 | 0 |
PprA-2 | 0.0418 | 0.5 |
PprA-3 | 0.0418 | 1.0 |
PprA-4 | 0.0418 | 2.0 |
PprA-5 | 0.0418 | 5.0 |
PprM-1 | 0.0454 | 0 |
PprM-2 | 0.0454 | 0.5 |
PprM-3 | 0.0454 | 1.0 |
PprM-4 | 0.0454 | 2.0 |
PprM-5 | 0.0454 | 5.0 |
RecA-1 | 0.0114 | 0 |
RecA-2 | 0.0114 | 0.5 |
RecA-3 | 0.0114 | 1.0 |
RecA-4 | 0.0114 | 2.0 |
RecA-5 | 0.0114 | 5.0 |
empty vector-1 | 0.0515 | 0 |
empty vector-2 | 0.0515 | 0.5 |
empty vector-3 | 0.0515 | 1.0 |
empty vector-4 | 0.0515 | 2.0 |
empty vector-5 | 0.0515 | 5.0 |
wild type-1 | 0.0546 | 0 |
wild type-2 | 0.0546 | 0.5 |
wild type-3 | 0.0546 | 1.0 |
wild type-4 | 0.0546 | 2.0 |
wild type-5 | 0.0546 | 5.0 |
September 20(Tue)
- To liquid culture:113,114
- colony check (cell survival)
Name | UV(mJ/cm^2) | conoly counts |
---|---|---|
PprI-1 | 0 | |
PprI-2 | 0.5 | |
PprI-3 | 1.0 | |
PprI-4 | 2.0 | |
PprI-5 | 5.0 | |
PprA-1 | 0 | |
PprA-2 | 0.5 | |
PprA-3 | 1.0 | |
PprA-4 | 2.0 | |
PprA-5 | 5.0 | |
PprM-1 | 0 | |
PprM-2 | 0.5 | |
PprM-3 | 1.0 | |
PprM-4 | 2.0 | |
PprM-5 | 5.0 | |
RecA-1 | 0 | |
RecA-2 | 0.5 | |
RecA-3 | 1.0 | |
RecA-4 | 2.0 | |
RecA-5 | 5.0 | |
Empty vector-1 | 0 | |
Empty vector-2 | 0.5 | |
Empty vector-3 | 1.0 | |
Empty vector-4 | 2.0 | |
Empty vector-5 | 5.0 | |
wild type-1 | 0 | |
wild type-2 | 0.5 | |
wild type-3 | 1.0 | |
wild type-4 | 2.0 | |
wild type-5 | 5.0 |
- Plating (cell survival)
Name | OD600 | UV(mJ/cm^2) |
---|---|---|
PprI-1 | 0.0109 | 0 |
PprI-2 | 0.0109 | 0.5 |
PprI-3 | 0.0109 | 1.0 |
PprI-4 | 0.0109 | 2.0 |
PprI-5 | 0.0109 | 5.0 |
PprA-1 | 0.0418 | 0 |
PprA-2 | 0.0418 | 0.5 |
PprA-3 | 0.0418 | 1.0 |
PprA-4 | 0.0418 | 2.0 |
PprA-5 | 0.0418 | 5.0 |
PprM-1 | 0.0454 | 0 |
PprM-2 | 0.0454 | 0.5 |
PprM-3 | 0.0454 | 1.0 |
PprM-4 | 0.0454 | 2.0 |
PprM-5 | 0.0454 | 5.0 |
RecA-1 | 0.0114 | 0 |
RecA-2 | 0.0114 | 0.5 |
RecA-3 | 0.0114 | 1.0 |
RecA-4 | 0.0114 | 2.0 |
RecA-5 | 0.0114 | 5.0 |
empty vector-1 | 0.0515 | 0 |
empty vector-2 | 0.0515 | 0.5 |
empty vector-3 | 0.0515 | 1.0 |
empty vector-4 | 0.0515 | 2.0 |
empty vector-5 | 0.0515 | 5.0 |
wild type-1 | 0.0546 | 0 |
wild type-2 | 0.0546 | 0.5 |
wild type-3 | 0.0546 | 1.0 |
wild type-4 | 0.0546 | 2.0 |
wild type-5 | 0.0546 | 5.0 |