Team:Osaka/week8

From 2011.igem.org

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==September 20(Tue)==
==September 20(Tue)==
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# colony check (cell survival)
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{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
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|+Table 8
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!Name!!UV(mJ/cm^2)!!conoly counts
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|-
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|PprI-1||0||
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|-
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|PprI-2||0.5||
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|-
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|PprI-3||1.0||
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|-
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|PprI-4||2.0||
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|-
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|PprI-5||5.0||
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|-
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|-
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|PprA-1||0||
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|-
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|PprA-2||0.5||
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|-
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|PprA-3||1.0||
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|-
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|PprA-4||2.0||
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|-
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|PprA-5||5.0||
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|-
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|PprM-1||0||
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|-
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|PprM-2||0.5||
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|-
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|PprM-3||1.0||
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|-
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|PprM-4||2.0||
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|-
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|PprM-5||5.0||
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|-
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|RecA-1||0||
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|-
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|RecA-2||0.5||
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|-
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|RecA-3||1.0||
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|-
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|RecA-4||2.0||
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|-
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|RecA-5||5.0||
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|-
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|Empty vector-1||0||
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|-
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|Empty vector-2||0.5||
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|-
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|Empty vector-3||1.0||
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|-
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|Empty vector-4||2.0||
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|-
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|Empty vector-5||5.0||
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|-
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|wild type-1||0||
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|-
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|wild type-2||0.5||
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|-
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|wild type-3||1.0||
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|-
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|wild type-4||2.0||
 +
|-
 +
|wild type-5||5.0||
 +
|}
==September 21(Wed)==
==September 21(Wed)==

Revision as of 13:12, 20 September 2011


Contents

September 18(Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19(Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 113(T):105(upstream)+110(downstream)+1-7A(vector)
    • 114(T):111(upstream)+112(downstream)+1-7A(vector)
  5. Transformation of ligation products
  6. Measurment of cell survival
Table 7
NameOD600UV(mJ/cm^2)
PprI0.01090 , 0.5 , 1 , 2 , 5
PprA0.04180 , 0.5 , 1 , 2 , 5
PprM0.04540 , 0.5 , 1 , 2 , 5
RecA0.01140 , 0.5 , 1 , 2 , 5
empty vector0.05150 , 0.5 , 1 , 2 , 5
wild type0.05460 , 0.5 , 1 , 2 , 5


September 20(Tue)

  1. colony check (cell survival)
Table 8
NameUV(mJ/cm^2)conoly counts
PprI-10
PprI-20.5
PprI-31.0
PprI-42.0
PprI-55.0
PprA-10
PprA-20.5
PprA-31.0
PprA-42.0
PprA-55.0
PprM-10
PprM-20.5
PprM-31.0
PprM-42.0
PprM-55.0
RecA-10
RecA-20.5
RecA-31.0
RecA-42.0
RecA-55.0
Empty vector-10
Empty vector-20.5
Empty vector-31.0
Empty vector-42.0
Empty vector-55.0
wild type-10
wild type-20.5
wild type-31.0
wild type-42.0
wild type-55.0

September 21(Wed)

September 22(Thu)

September 23(Fri)

September 24(Sat)

Back to Notebook

Retrieved from "http://2011.igem.org/Team:Osaka/week8"