Team:Valencia/Project1

Colicin G and Colicin H

Colicins are proteins produced by some strains of Escherichia coli that are lethal for related strains of E. coli. Colicins of strain CA46 (colicin G producer) and of strain CA58 (colicin H producer) should be the same, and indeed, the sensitivity patterns were nearly identical. Only the triple mutant receptors fepA cir fiu was completely resistant to the colicins from these strains.

(i) the production of colicin by colicinogenic E. coli cells is induced by SOS agents, as is seen with lysogenic phages, and is lethal for producing cells.

(ii) the produced colicin is released into the medium late after synthesis (later shown not to be the case for all colicins).

(iii) colicin kills sensitive cells according to single-hit kinetics.

(iv) colicin is not active against the producing bacteria if there is presence of a specific antagonist protein called the immunity protein.

All colicins are organized into three domains, each corresponding to one step of colicin action:

o The N-terminal domain is involved in translocation through the membrane.

o The central domain is involved in binding to the receptor.

o The C-terminal domain contains the active part.

Cascales E. et al. Colicin Biology. Microbiol Mol Biol Rev. 2007 March; 71(1): 158–229.

The sequence of the colicin G and colicin H determinants have been deposited under the accession numbers AJ515251 and AJ515252, respectively, in the EMBL/GenBank database.

Escherichia coli microcin operon, strain CA46 (CA46 ColG) [http://www.ncbi.nlm.nih.gov/nuccore/AJ515251]

Escherichia coli microcin operon, strain CA58 (CA58 ColH) [http://www.ncbi.nlm.nih.gov/nuccore/AJ515252]

Microcins

Microcins are a peculiar class of gene-encoded low-molecular-mass antibacterial peptides secreted by enterobacteria. They contribute to the regulation of microbial competitions within the intestinal microbiota. The genetic systems involved in microcin biosynthesis share a conserved organization. Similar to bacteriocins, microcins exert potent antibacterial activity directed against phylogenetically-related bacterial strains, with minimal inhibitory concentrations in the nanomolar range.

Usually, microcins exhibit "Trojan horse" mechanisms of antibacterial activity: some microcin structures are a mime of essential elements, permitting recognition by outer membrane receptors used for vital functions in bacteria and further translocation into the periplasmic space, other microcins are secreted as harmless molecules and further processed in susceptible bacteria to form the toxic entity. When inside target bacteria, microcins bind essential enzymes or interact with the inner membrane to form a bacterial killing structure.

Microcin C51

Microcin C51 is a nucleopeptide of 1.18 kDa molecular mass and the first described nucleopeptide antibiotic. It contains a heptapeptide with the N-terminal formylmethionine and C-terminal asparagine bound to AMP via an aliphatic chain. Microcine C51 is characterized by a broad spectrum of action. Expression in Escherichia coli is activated upon decelerated growth of cells during their transition to the stationary growth phase. This synthesis is controlled by four genes organized in an operon.