Team:Osaka/week8

From 2011.igem.org

(Difference between revisions)
Line 22: Line 22:
!Name!!OD600!!UV(mJ/cm^2)
!Name!!OD600!!UV(mJ/cm^2)
|-
|-
-
|PprI①||0.0109||0
+
|PprI||0.0109||0,0.5,1,2,5
|-
|-
-
|PprI②||0.0109||0.5
+
|PprA||0.0418||0,0.5,1,2,5
|-
|-
-
|PprI③||0.0109||1
+
|PprM||0.0454||0,0.5,1,2,5
|-
|-
-
|PprI④||0.0109||2
+
|RecA||0.0114||0,0.5,1,2,5
|-
|-
-
|PprI⑤||0.0109||5
+
|empty vector||0.0515||0,0.5,1,2,5
|-
|-
-
|PprA||400μl||2h
+
|wild type||0.0546||0,0.5,1,2,5
-
|-
+
-
|PprM||400μl||1h
+
-
|-
+
-
|RecA||40μl||2h
+
-
|-
+
-
|empty vector||40μl||1h
+
-
|-
+
-
|wild type||40μl||1h
+
|}
|}

Revision as of 02:20, 20 September 2011


Contents

September 18(Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19(Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 113(T):105(upstream)+110(downstream)+1-7A(vector)
    • 114(T):111(upstream)+112(downstream)+1-7A(vector)
  5. Transformation of ligation products
  6. Measurment of cell survival
Table 7
NameOD600UV(mJ/cm^2)
PprI0.01090,0.5,1,2,5
PprA0.04180,0.5,1,2,5
PprM0.04540,0.5,1,2,5
RecA0.01140,0.5,1,2,5
empty vector0.05150,0.5,1,2,5
wild type0.05460,0.5,1,2,5


September 20(Tue)

September 21(Wed)

September 22(Thu)

September 23(Fri)

September 24(Sat)

Back to Notebook

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