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Team:UPO-Sevilla/Project/Improving Flip Flop/Modeling/Introduction
From 2011.igem.org
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<p>The Repressor production will be also modeled using Michaelis-Menten kinetics:</p> | <p>The Repressor production will be also modeled using Michaelis-Menten kinetics:</p> | ||
| - | <p><strong>REPRESSION:</strong | + | <p><strong>REPRESSION:</strong> Promoter + n · Repressor → Promoter_Repressor<sub>n</sub></p> |
| - | <p></p> | + | <p>Finally we use a Hill kinetics model for the transcription inhibition, which offers an approximation to the cooperative action.</p> |
| - | <p></p> | + | <p>And in addition to this, we have to include the new reaction involved in the new system. These are:</p> |
| - | <p></p> | + | <p><strong>PROTEOLYSIS: </strong> Protease + Repressor 1 → Protease</p> |
| - | <p></p> | + | <p>We will consider a mass action rate law, where the protein will be digested according to the protease concentration.</p> |
| - | + | ||
| + | <p><strong>IHNIBITION of TRANSLATION: </strong> asRNA + mRNA → RNA complex</p> | ||
| + | |||
| + | <p>We assume a mass action rate law again. | ||
| + | </p> | ||
| + | |||
| + | <p>These two new effects may make the toggle switch more robust. Due to the protease is expressed at the same rate level than </p> | ||
| + | |||
| + | <h2>Diagram</h2> | ||
| + | |||
| + | <div class="center"> | ||
| + | <img src="https://static.igem.org/mediawiki/2011/1/1c/UPOSevillaDiagramMB.png" alt="Diagram MB" /> | ||
| + | </div> | ||
| + | |||
| + | <p>Here we can see all the species involved in the new model for the bistable.</p> | ||
</div> | </div> | ||
Revision as of 00:06, 20 September 2011
Modeling
Introduction
In this chapter we model the modified toggle switch
To start, we include to this analysis the processes described at ‘Basic Bistable – Modeling’.
TRANSCRIPTION: RNAp + Promoter → RNAp + Promoter + mRNA
The mRNA production will be described using Michaelis-Menten kinetics:
TRANSLATION: mRNA + Ribosomes → mRNA + Ribosomes + Repressor
The Repressor production will be also modeled using Michaelis-Menten kinetics:
REPRESSION: Promoter + n · Repressor → Promoter_Repressorn
Finally we use a Hill kinetics model for the transcription inhibition, which offers an approximation to the cooperative action.
And in addition to this, we have to include the new reaction involved in the new system. These are:
PROTEOLYSIS: Protease + Repressor 1 → Protease
We will consider a mass action rate law, where the protein will be digested according to the protease concentration.
IHNIBITION of TRANSLATION: asRNA + mRNA → RNA complex
We assume a mass action rate law again.
These two new effects may make the toggle switch more robust. Due to the protease is expressed at the same rate level than
Diagram
Here we can see all the species involved in the new model for the bistable.
Sponsors
