Team:Paris Bettencourt/Data

From 2011.igem.org

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<h2>The YFP concentration system : YFP:tetR and TetO array</h2>
<h2>The YFP concentration system : YFP:tetR and TetO array</h2>
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<center><img src="https://static.igem.org/mediawiki/2011/6/62/Tetdata.jpg">
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</html>
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<p><u>Fig1:</u> Schematics of the YFP concentration design</center></p>  
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[[File:YFP_concentration_principle.jpg|center|950px|The YFP concentration system principle]]
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<html>
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<h3>Data for our favorite new parts</h3>
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</html>
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# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
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# [http://partsregistry.org/Part:BBa_K606003 Main Page] - '''Multihost replicative plasmid pHM3, BBa_K606003''': This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
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# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
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<html>
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<h3>Data for Pre-existing Parts</h3>
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</html>
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# [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - '''pVeg + SpoVG, BBa_K143053''' (Imperial College, iGEM 2008):
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<html>
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<br/>
<br/>
<br/>
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<h2>The T7 polymerase diffusion system</h2>
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</html>
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[[File:T7_pol_diff.jpg|center|950px|The T7 polymerase diffusion system principle]]
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<html>
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<h3>Data for Pre-existing Parts</h3>
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</html>
<h3>Data for our favorite new parts</h3>
<h3>Data for our favorite new parts</h3>
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<br/>
<br/>
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<h2>The tRNA Amber diffusion system</h2>
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</html>
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[[File:tRNA_amber_principle.jpg|center|950px|The tRNA Amber diffusion system principle]]
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<html>
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<h3>Data for Pre-existing Parts</h3>
 +
</html>
 +
 +
<h3>Data for our favorite new parts</h3>
 +
</html>
 +
# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
 +
# [http://partsregistry.org/Part:BBa_K606003 Main Page] - '''Multihost replicative plasmid pHM3, BBa_K606003''': This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
 +
# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
 +
<html>
 +
 +
<h3>Data for Pre-existing Parts</h3>
 +
</html>
 +
# [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - '''pVeg + SpoVG, BBa_K143053''' (Imperial College, iGEM 2008):
 +
<html>
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<br/>
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<br/>
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<h2>The ComS diffusion system</h2>
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</html>
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[[File:coms_diffusion_principle.jpg|center|950px|The ComS diffusion system principle]]
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<html>
 +
 +
<h3>Data for Pre-existing Parts</h3>
 +
</html>
 +
 +
<h3>Data for our favorite new parts</h3>
 +
</html>
 +
# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
 +
# [http://partsregistry.org/Part:BBa_K606003 Main Page] - '''Multihost replicative plasmid pHM3, BBa_K606003''': This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
 +
# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
 +
<html>
 +
 +
<h3>Data for Pre-existing Parts</h3>
 +
</html>
 +
# [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - '''pVeg + SpoVG, BBa_K143053''' (Imperial College, iGEM 2008):
 +
<html>
 +
<br/>
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<br/>
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Revision as of 18:33, 19 September 2011

Team IGEM Paris 2011

Data page

The YFP concentration system : YFP:tetR and TetO array

The YFP concentration system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The T7 polymerase diffusion system

The T7 polymerase diffusion system principle

Data for Pre-existing Parts

Data for our favorite new parts

</html>

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The tRNA Amber diffusion system

The tRNA Amber diffusion system principle

Data for Pre-existing Parts

Data for our favorite new parts

</html>

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The ComS diffusion system

The ComS diffusion system principle

Data for Pre-existing Parts

Data for our favorite new parts

</html>

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



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