"
Team:Paris Bettencourt/Designs/List
From 2011.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
<h1>Design List</h1> | <h1>Design List</h1> | ||
| - | + | <h2>Receptor/amplificator design</h2> | |
| + | <p>These designs rely on synthetic biology constructs as receptor and amplificator.</p> | ||
<table> | <table> | ||
<tr> | <tr> | ||
| Line 24: | Line 25: | ||
</td> | </td> | ||
</tr> | </tr> | ||
| + | </table> | ||
| + | <h2>Using bistable switches</h2> | ||
| + | <p>We noticed that using natural or artificial bistable switches in the receiving cell gave us a receptor and an auto amplifier. One molecule carefully chosen could toggle the switch in another position. All we have to do is see if it diffuses through the nanotubes. | ||
| + | <table> | ||
<tr> | <tr> | ||
<td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/ComS_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a> | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/ComS_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a> | ||
| Line 30: | Line 35: | ||
</td> | </td> | ||
</tr> | </tr> | ||
| - | + | <tr> | |
| + | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/SinOp"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a> | ||
| + | </td> | ||
| + | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/SinOp">Sin Operon</a></b> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Lambda_switch"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a> | ||
| + | </td> | ||
| + | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Lambda_switch">ComS diffusion</a></b> We took advantage of a switch already existing in <i>B.Subtilis</i> (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes. | ||
| + | </td> | ||
| + | </tr> | ||
</table> | </table> | ||
Revision as of 17:33, 19 September 2011
Design List
Receptor/amplificator design
These designs rely on synthetic biology constructs as receptor and amplificator.
|
YFP concentration This design relies on a TetO-array which allow us to concentrate YFP-TetR fusion proteins. |
|
T7 RNA polymerase diffusion In this design, we introduce the use of the T7 polymerase both as the transfer molecule and as the auto-amplification system. |
|
tRNA amber diffusion The tRNA amber is the smallest molecule we are trying to get pass the nanotubes. |
Using bistable switches
We noticed that using natural or artificial bistable switches in the receiving cell gave us a receptor and an auto amplifier. One molecule carefully chosen could toggle the switch in another position. All we have to do is see if it diffuses through the nanotubes.
|
ComS diffusion We took advantage of a switch already existing in B.Subtilis (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes. |
|
|
Sin Operon |
|
|
ComS diffusion We took advantage of a switch already existing in B.Subtilis (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes. |
