Team:Paris Bettencourt/Experiments

From 2011.igem.org

(Difference between revisions)
Line 3: Line 3:
<h1>Experiments</h1>
<h1>Experiments</h1>
<p>You can find here all the experiments we did in the lab! The data is organized by design and you will be able to find both the characterization steps and our diffusion experiments.</p>
<p>You can find here all the experiments we did in the lab! The data is organized by design and you will be able to find both the characterization steps and our diffusion experiments.</p>
-
 
+
<h2>Preliminaries experiments</h2>
<table>
<table>
<tr>
<tr>
Line 11: Line 11:
   </td>
   </td>
</tr>
</tr>
 +
</table>
 +
<h2>Our major designs</h2>
 +
<table>
<tr>
<tr>
-
   <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Atb_exp"><img style="width:150px" src="https://static.igem.org/mediawiki/2011/2/2b/Question_mark_button.png"></a>
+
   <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion"><img style="width:150px" src="https://static.igem.org/mediawiki/2011/d/d0/YFP_concentration_button.png"></a>
   </td>
   </td>
-
   <td><p><b>The <a href="https://2011.igem.org/Team:Paris_Bettencourt/Atb_exp">antibiotic resistance exchange</a></b> is a more tricky experiment in which bacteria are shown to exchange resistance enzyme through nanotube and allow the population to survive even though all the cells does not carry the resistence.We invite you to <a href="https://2011.igem.org/Team:Paris_Bettencourt/Atb_exp">visit corresponding the page</a> to learn more about what we did and the results we had.</p>
+
   <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion">YFP concentration</a></b>: this design relies on a TetO-array which allow us to concentrate YFP-TetR fusion proteins.
   </td>
   </td>
</tr>
</tr>
 +
<tr>
 +
  <td style="width:200px; text-align:center;"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/e/e4/T7_button.png"></a>
 +
  </td>
 +
  <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion">T7 RNA polymerase diffusion</a></b>: in this design, we used the T7 polymerase both as the transfer molecule and as the auto-amplification system.
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/tRNA_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/5/53/TRNAamber-button.png"></a>
 +
  </td>
 +
  <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/tRNA_diffusion">tRNA amber diffusion</a></b>: the tRNA amber is the smallest molecule we are trying to get pass the nanotubes.
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/ComS_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a>
 +
  </td>
 +
  <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/ComS_diffusionn">ComS diffusion</a></b>: we took advantage of a switch already existing in <i>B.Subtilis</i> (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes.
 +
  </td>
 +
</tr>
 +
</table>
</table>

Revision as of 15:59, 19 September 2011

Team IGEM Paris 2011

Experiments

You can find here all the experiments we did in the lab! The data is organized by design and you will be able to find both the characterization steps and our diffusion experiments.

Preliminaries experiments

Preliminaries experiments: go back and see how we re-did the experiment of the original nanotubes paper.

Our major designs

YFP concentration: this design relies on a TetO-array which allow us to concentrate YFP-TetR fusion proteins.
T7 RNA polymerase diffusion: in this design, we used the T7 polymerase both as the transfer molecule and as the auto-amplification system.
tRNA amber diffusion: the tRNA amber is the smallest molecule we are trying to get pass the nanotubes.
ComS diffusion: we took advantage of a switch already existing in B.Subtilis (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes.

Retrieved from "http://2011.igem.org/Team:Paris_Bettencourt/Experiments"