Team:EPF-Lausanne/Our Project/T7 promoter variants

From 2011.igem.org

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(Characterization with RFP)
(Characterization with RFP)
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With such a system in place, the transformation of our T7 and T7-lac promoter variant plasmids into BL21 cells would produce little to no reporter expression. Now to induce promoter activity, we rely on the fact that the chemical IPTG blocks the repressor action of LacI.  
With such a system in place, the transformation of our T7 and T7-lac promoter variant plasmids into BL21 cells would produce little to no reporter expression. Now to induce promoter activity, we rely on the fact that the chemical IPTG blocks the repressor action of LacI.  
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[[File:bl21_induction_start.png]]
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[[File:bl21_induction_start.png|300 px]]
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[[File:bl21_induction_complete.png]]
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[[File:bl21_induction_complete.png|300px]]
=== DNA Recovery with Lysis ===
=== DNA Recovery with Lysis ===

Revision as of 10:52, 19 September 2011