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Team:Paris Bettencourt/GFP diff
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<h1>GFP diffusion</h1> | <h1>GFP diffusion</h1> | ||
| - | <p>The keystone experiment of the Dubey and Ben-Yehuda paper <a href="http://bms.ucsf.edu/sites/ucsf-bms.ixm.ca/files/marjordan_06022011.pdf">[1]</a> is a simple experiment. | + | <h2>The original experiment</h2> |
| + | <p>The keystone experiment of the Dubey and Ben-Yehuda paper <a href="http://bms.ucsf.edu/sites/ucsf-bms.ixm.ca/files/marjordan_06022011.pdf">[1]</a> is a simple experiment. We use one strain of <i>gfp + B.subtilis</i> and one <i>gfp - B.subtilis</i> strain. The two strains of <i>B.subtilis</i> are put together (ratio 1:1) on an LB-agarose (1.5%) plate in exponantial phase. The goal is to have a <em>monolayer of densely packed cells</em>, which is not that easy.</p> | ||
| + | <p>The plated bacteria are then observed through fluorescent microscopy. After a while (between 15min and 2 hours), a transfer of GFP can be observed from the <i>gfp +</i> cells towards the <i>gfp-</i> cells. This cell-to-cell communication was previously unheard of and the original paper <a href="http://bms.ucsf.edu/sites/ucsf-bms.ixm.ca/files/marjordan_06022011.pdf">[1]</a> strongly suggest that the so-called nanotubes observed through electronic microscopy by the authors is the reason of this transfer. | ||
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Revision as of 15:03, 17 September 2011
GFP diffusion
The original experiment
The keystone experiment of the Dubey and Ben-Yehuda paper [1] is a simple experiment. We use one strain of gfp + B.subtilis and one gfp - B.subtilis strain. The two strains of B.subtilis are put together (ratio 1:1) on an LB-agarose (1.5%) plate in exponantial phase. The goal is to have a monolayer of densely packed cells, which is not that easy.
The plated bacteria are then observed through fluorescent microscopy. After a while (between 15min and 2 hours), a transfer of GFP can be observed from the gfp + cells towards the gfp- cells. This cell-to-cell communication was previously unheard of and the original paper [1] strongly suggest that the so-called nanotubes observed through electronic microscopy by the authors is the reason of this transfer.
