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Team:Washington/Alkanes/Future
From 2011.igem.org
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::Our current in vivo system only efficiently makes C15 alkanes. To be efficient enough for factory production, there are two broad goals to be done: 1. increase production efficiency 2. Diversity the range of alkanes for the system. 3. Increase scale of system for industrial processes. We have already begun efforts to expand the efficiency and scope of alkane production. | ::Our current in vivo system only efficiently makes C15 alkanes. To be efficient enough for factory production, there are two broad goals to be done: 1. increase production efficiency 2. Diversity the range of alkanes for the system. 3. Increase scale of system for industrial processes. We have already begun efforts to expand the efficiency and scope of alkane production. | ||
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| + | [https://2011.igem.org/Team:Washington/Alkanes/Future/Vector System Optimization] | ||
| + | :<nowiki> The efficiency of this system was reported to be much higher than our initial system, but the growth conditions of the assay and the DNA was not available to us. We increased production efficiency by altering the initial environmental conditions in the assay.</nowiki> | ||
[https://2011.igem.org/Team:Washington/Alkanes/Future/DecarbDesign Decarbonylase Redesign] | [https://2011.igem.org/Team:Washington/Alkanes/Future/DecarbDesign Decarbonylase Redesign] | ||
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:<nowiki> Our system is only capable of producing unbranched alkanes, as the cell mainly utilizes straight chained fatty acids. However, fuel we use are also composed largely of branched alkanes that affect very important properties of the fuel such as flash point and freezing point. If our fuels are truly intended to be synthesized in bacteria, we need to work on methods of making those crucial branched chained alkanes. We explored FabH2, a protein that when involved in fatty acid synthesis makes branched fatty acids. </nowiki> | :<nowiki> Our system is only capable of producing unbranched alkanes, as the cell mainly utilizes straight chained fatty acids. However, fuel we use are also composed largely of branched alkanes that affect very important properties of the fuel such as flash point and freezing point. If our fuels are truly intended to be synthesized in bacteria, we need to work on methods of making those crucial branched chained alkanes. We explored FabH2, a protein that when involved in fatty acid synthesis makes branched fatty acids. </nowiki> | ||
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[https://2011.igem.org/Team:Washington/Alkanes/Future/Localization Enzyme Localization] | [https://2011.igem.org/Team:Washington/Alkanes/Future/Localization Enzyme Localization] | ||
Revision as of 03:55, 16 September 2011
- Our current in vivo system only efficiently makes C15 alkanes. To be efficient enough for factory production, there are two broad goals to be done: 1. increase production efficiency 2. Diversity the range of alkanes for the system. 3. Increase scale of system for industrial processes. We have already begun efforts to expand the efficiency and scope of alkane production.
- The efficiency of this system was reported to be much higher than our initial system, but the growth conditions of the assay and the DNA was not available to us. We increased production efficiency by altering the initial environmental conditions in the assay.
- One way to diversify the kind of alkanes produced is to alter the substrate specificity of the proteins involved. We decided to mutate the aldehyde decarbonylase to produce shorter-chain alkanes.
- Another way to diversify our system is to use alternative proteins. Our current system uses acyl-ACP reductase, and we've identified an hypothetical alternative system that produces aldehydes: LuxCDE, made from parts from the 2010
- Our system is only capable of producing unbranched alkanes, as the cell mainly utilizes straight chained fatty acids. However, fuel we use are also composed largely of branched alkanes that affect very important properties of the fuel such as flash point and freezing point. If our fuels are truly intended to be synthesized in bacteria, we need to work on methods of making those crucial branched chained alkanes. We explored FabH2, a protein that when involved in fatty acid synthesis makes branched fatty acids.
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