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Team:Washington/Magnetosomes/Results
From 2011.igem.org
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| - | === | + | ===Magnetosome Toolkit:=== |
| - | + | ||
| + | Using the vectors we created in our Gibson Assembly Toolkit, our team was able to create a "Magnetosome Toolkit" consisting of the most basic parts required for future Magnetosome gene manipulation. | ||
| + | |||
| + | After designing appropriate primers and using Standard PCR, and our modified Gibson Protocol, we were able to successfully extract out the following Magnetosome genes: | ||
| + | *mamHI | ||
| + | *mamE | ||
| + | *mamJ | ||
| + | *mamKL | ||
| + | *mamQRB | ||
| + | *mamSTU | ||
| + | *mamV | ||
| + | |||
| + | These genes were visualized on gels and sequence confirmed. However, in order to make sure that gene-activity had not be lost, we created mam gene-sfGFP fusions, transformed them into E.coli, and imaged them with microscopy. The two most notable constructs were mamK-sfGFP and mamI-sfGFP | ||
| + | |||
| + | (--> we're going to put pictures here....<--) | ||
Revision as of 00:22, 16 September 2011
Magnetosome Toolkit:
Using the vectors we created in our Gibson Assembly Toolkit, our team was able to create a "Magnetosome Toolkit" consisting of the most basic parts required for future Magnetosome gene manipulation.
After designing appropriate primers and using Standard PCR, and our modified Gibson Protocol, we were able to successfully extract out the following Magnetosome genes:
- mamHI
- mamE
- mamJ
- mamKL
- mamQRB
- mamSTU
- mamV
These genes were visualized on gels and sequence confirmed. However, in order to make sure that gene-activity had not be lost, we created mam gene-sfGFP fusions, transformed them into E.coli, and imaged them with microscopy. The two most notable constructs were mamK-sfGFP and mamI-sfGFP
(--> we're going to put pictures here....<--)
