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Team:Washington/Protocols/Overnights.
From 2011.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
# Obtain a 14 mL Sterile Polypropylene Round-Bottom Tube. | # Obtain a 14 mL Sterile Polypropylene Round-Bottom Tube. | ||
# Add ~ 3-5 mLs of an appropriate broth (either LB or TB) | # Add ~ 3-5 mLs of an appropriate broth (either LB or TB) | ||
| - | # | + | # Add an appropriate antibiotic if necessary and set this tube safely aside |
| + | #* Amp (1:100) | ||
| + | #* Chlor (1:1000) | ||
| + | #* Kan (1:1000) | ||
| + | # Prick the desired colony off a plate and suspend it in 50 uL PBS. | ||
| + | #* Refer to the PBS stock making protocol! | ||
| + | # Add 10 uL of the PBS sample and add it to the broth previously set aside. | ||
| + | #* Incubate overnight cultures @ 37oC, overnight with shaking. | ||
Latest revision as of 18:13, 15 September 2011
Preparation of Overnight Cultures
- Obtain a 14 mL Sterile Polypropylene Round-Bottom Tube.
- Add ~ 3-5 mLs of an appropriate broth (either LB or TB)
- Add an appropriate antibiotic if necessary and set this tube safely aside
- Amp (1:100)
- Chlor (1:1000)
- Kan (1:1000)
- Prick the desired colony off a plate and suspend it in 50 uL PBS.
- Refer to the PBS stock making protocol!
- Add 10 uL of the PBS sample and add it to the broth previously set aside.
- Incubate overnight cultures @ 37oC, overnight with shaking.
