Team:Paris Bettencourt/Data

From 2011.igem.org

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<h2>Model and experiments</h2>
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<h2>Data for our favorite new parts</h2>
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# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
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# [http://partsregistry.org/Part:BBa_K606003 Main Page] - '''Multihost replicative plasmid pHM3, BBa_K606003''': This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
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# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
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<p>To know more about what we have done on this system and in the experiments, we invite you to visit the corresponding <em>diffusion modeling</em> and <em>experiment</em> pages:</p>
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<h2>Data for our favorite new parts</h2>
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<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Modeling/Diffusion">Diffusion modeling</a></li>
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# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
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<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion">Experiments</a></li>
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# [http://partsregistry.org/Part:BBa_K606003 Main Page] - '''Multihost replicative plasmid pHM3, BBa_K606003''': This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
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</ul>
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# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
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<h2>Data for Pre-existing Parts</h2>
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</html>
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# [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - '''pVeg + SpoVG, BBa_K143053''' (Imperial College, iGEM 2008):  
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Revision as of 13:27, 15 September 2011

Team IGEM Paris 2011

Data page

The YFP concentration system : YFP:tetR and TetO array

Fig1: Schematics of the YFP concentration design


Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):