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Team:Paris Bettencourt/tRNA diffusion
From 2011.igem.org
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| + | <h1>The tRNA amber diffusion</h1> | ||
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| + | <p>What we call a <em>tRNA amber</em> is a transfer RNA which anticodon is complementary with a stop codon and especially with an amber stop codon i.e. UAG. Our idea was to use the nanotubes to allow a receiver cell to translate a mRNA containing an amber mutation. The emitter cell will produce this mutated transfer RNA (we mutated a YtRNA for it to recognise the stop codon) and the receiver cell will then be able to translate a protein which gene contain an amber mutation. This protein will part of a reporter system.</p> | ||
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| + | We summed up this principle in the scheme below: | ||
[[File:tRNA_Amber_principle.jpg|thumb|center|upright=3.0|The tRNA Amber supressor design principle]] | [[File:tRNA_Amber_principle.jpg|thumb|center|upright=3.0|The tRNA Amber supressor design principle]] | ||
Revision as of 13:10, 15 September 2011
The tRNA amber diffusion
What we call a tRNA amber is a transfer RNA which anticodon is complementary with a stop codon and especially with an amber stop codon i.e. UAG. Our idea was to use the nanotubes to allow a receiver cell to translate a mRNA containing an amber mutation. The emitter cell will produce this mutated transfer RNA (we mutated a YtRNA for it to recognise the stop codon) and the receiver cell will then be able to translate a protein which gene contain an amber mutation. This protein will part of a reporter system.
We summed up this principle in the scheme below: [[File:tRNA_Amber_principle.jpg|thumb|center|upright=3.0|The tRNA Amber supressor design principle]] We are now designing precisely the primers and the missing bricks.
