Team:UT-Tokyo/Data/Method

From 2011.igem.org

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|style="text-align:right"|100ml
|style="text-align:right"|100ml
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*SOB broth
*SOB broth
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Before use, 100× 1Mg sol.
Before use, 100× 1Mg sol.
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{|
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|MgCl2
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|500mM
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|-
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|MgSO4
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|500mM
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|}
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*M9
*M9

Revision as of 12:54, 15 September 2011

Reagents

  • LB broth
reagents final conc. amount
Bacto trypton 1% 1g
NaCl 0.5% 0.5g
Yeast extracs 0.5% 0.5g
MilliQ - 100ml
Total 100ml


  • SOB broth
reagents final conc. amount
Bacto trypton 2% 20g
NaCl 0.05% 0.5g
Yeast extracs 0.5% 5g
KCl (250mM) 2.5mM 10ml
MilliQ - 1000ml
Total 1L

Before use, 100× 1Mg sol.

MgCl2 500mM
MgSO4 500mM


  • M9
  • 50× TAE
  • TB


Cultures

Extraction of DNA from cells

Amplification of DNA

Analysis of DNA

Assays

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