Team:UT-Tokyo/Data/Method

From 2011.igem.org

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==Reagents==
==Reagents==
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<div style="float:left">
*LB broth
*LB broth
{|
{|
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|style="text-align:right"|100ml
|style="text-align:right"|100ml
|}
|}
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</div>
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<div style="margin-left: 487.5px;">
*SOB broth
*SOB broth
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*M9
*M9
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</div>
 +
<div style="clear:both">
*50&times; TAE
*50&times; TAE
 +
</div>
*TB
*TB

Revision as of 11:44, 15 September 2011

Reagents

  • LB broth
reagents final conc. amount
Bacto trypton 1% 1g
NaCl 0.5% 0.5g
Yeast extracs 0.5% 0.5g
MilliQ - 100ml
Total 100ml
  • SOB broth
  • M9
  • 50× TAE
  • TB


Cultures

Extraction of DNA from cells

Amplification of DNA

Analysis of DNA

Assays

Retrieved from "http://2011.igem.org/Team:UT-Tokyo/Data/Method"