"
Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy
From 2011.igem.org
(Difference between revisions)
| Line 53: | Line 53: | ||
<p style="line-height : 1.5em"> | <p style="line-height : 1.5em"> | ||
| - | <b> | + | <b>5.</b> Observe the slides with a fluorescent microscope. |
</p> | </p> | ||
Revision as of 11:24, 15 September 2011
Microscopy test
This protocol aims at
Procedure
1. In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.
2. Add 100µL of bacteria from a saturated liquid culture.
3. Finally, add 3 or 4 sterile glass slides with a sterile tweezer.
4. Incubate at 30°C overnight.
5. Observe the slides with a fluorescent microscope.
