Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy
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+ | In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.<br/> | ||
+ | |||
+ | Add 100µL of bacteria from a saturated liquid culture. <br/> | ||
+ | |||
+ | Finally, add 3 or 4 sterile glass slides with a sterile tweezer. <br/> | ||
+ | |||
+ | Incubate at 30°C overnight. <br/><br/> | ||
+ | |||
+ | |||
+ | |||
+ | Observe the slides with a fluorescent microscope. | ||
</div> | </div> |
Revision as of 11:18, 15 September 2011
In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.
Add 100µL of bacteria from a saturated liquid culture.
Finally, add 3 or 4 sterile glass slides with a sterile tweezer.
Incubate at 30°C overnight.
Observe the slides with a fluorescent microscope.
Add 100µL of bacteria from a saturated liquid culture.
Finally, add 3 or 4 sterile glass slides with a sterile tweezer.
Incubate at 30°C overnight.
Observe the slides with a fluorescent microscope.