Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy

From 2011.igem.org

(Difference between revisions)
(Created page with "{{Lyon-INSA-ENS/header}} {{Lyon-INSA-ENS/blocStyle}} {{INSA-Lyon/styletestaurelie}} {{Lyon-INSA-ENS/menuhorizontal}} {{Lyon-INSA-ENS/menuNotebookProtocoles}} <html> <div class=...")
Line 8: Line 8:
<div class="contenugrand2";>  
<div class="contenugrand2";>  
 +
 +
In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.<br/>
 +
 +
Add 100µL of bacteria from a saturated liquid culture. <br/>
 +
 +
Finally, add 3 or 4 sterile glass slides with a sterile tweezer. <br/>
 +
 +
Incubate at 30°C overnight. <br/><br/>
 +
 +
 +
 +
Observe the slides with a fluorescent microscope.
</div>
</div>

Revision as of 11:18, 15 September 2011



In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.
Add 100µL of bacteria from a saturated liquid culture.
Finally, add 3 or 4 sterile glass slides with a sterile tweezer.
Incubate at 30°C overnight.

Observe the slides with a fluorescent microscope.




ENS assystem Biomérieux INSA INSA