Team:UT-Tokyo/Data/Method

From 2011.igem.org

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Line 13: Line 13:
|align="right"|1g
|align="right"|1g
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|-
-
|NaCl
 
-
|align="right"|0.5%
 
-
|align="right"|0.5g
 
-
|-
 
-
|Yeast extracs
 
-
|align="right"|0.5%
 
-
|align="right"|0.5g
 
-
|-
 
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|MilliQ
 
-
|align="right"|
 
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|align="right"|100ml
 
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|-
 
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ってなってるんですが、どうしたものか…。
ってなってるんですが、どうしたものか…。
Line 41: Line 28:
|align="right"|1g
|align="right"|1g
|-
|-
 +
|NaCl
 +
|align="right"|0.5%
 +
|align="right"|0.5g
 +
|-
 +
|Yeast extracs
 +
|align="right"|0.5%
 +
|align="right"|0.5g
 +
|-
 +
|MilliQ
 +
|align="right"|
 +
|align="right"|100ml
 +
|-
 +
|}
|}

Revision as of 01:37, 15 September 2011

Reagents

  • LB broth
reagents final conc. amount
Bacto trypton 1% 1g
NaCl 0.5% 0.5g
Yeast extracs 0.5% 0.5g
MilliQ 100ml


  • reagents
    East extracts
  • SOB broth
  • M9
  • 50× TAE
  • TB


Cultures

Extraction of DNA from cells

Amplification of DNA

Analysis of DNA

Assays